Nature Genetics 37, 1270 - 1273 (2005)
Published online: 16 October 2005; | doi:10.1038/ng1659
A mutation in Sec15l1 causes anemia in hemoglobin deficit (hbd) miceJackie E Lim1, 2, Ou Jin1, Carolyn Bennett1, 2, Kelly Morgan3, Fudi Wang1, 2, Cameron C Trenor III1, 2, Mark D Fleming2, 4
& Nancy C Andrews1, 2, 51
Division of Hematology/Oncology, Children's Hospital Boston, Karp Family Research Laboratories RM 8-125, Boston, Massachusetts 02115-5737 USA. 2
Harvard Medical School, Boston, Massachusetts, USA. 3
Division of Hematology, Brigham and Women's Hospital, Boston, Massachusetts, USA. 4
Department of Pathology, Children's Hospital Boston, Boston, Massachusetts, USA. 5
Howard Hughes Medical Institute, Boston, Massachusetts, USA.
Correspondence should be addressed to Nancy C Andrews nancy_andrews@hms.harvard.edu Hemoglobin deficit (hbd) mice carry a spontaneous mutation that impairs erythroid iron assimilation but does not cause other defects. Normal delivery of iron to developing erythroid precursors is highly dependent on the transferrin cycle. Through genetic mapping and complementation experiments, we show that the hbd mutation is an in-frame deletion of a conserved exon of the mouse gene Sec15l1, encoding one of two Sec15 proteins implicated in the mammalian exocyst complex. Sec15l1 is linked to the transferrin cycle through its interaction with Rab11, a GTPase involved in vesicular trafficking. We propose that inactivation of Sec15l1 alters recycling of transferrin cycle endosomes and increases the release of transferrin receptor exocytic vesicles. This in turn decreases erythroid iron uptake. Determining the molecular basis of the hbd phenotype provides new insight into the intricate mechanisms necessary for normal erythroid iron uptake and the function of a mammalian exocyst protein.
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