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Commentary
Nature Genetics  36, 921 - 924 (2004)
doi:10.1038/ng0904-921

The Knockout Mouse Project

The Comprehensive Knockout Mouse Project Consortium: Christopher P Austin1, James F Battey2, Allan Bradley3, Maja Bucan4, Mario Capecchi5, Francis S Collins6, William F Dove7, Geoffrey Duyk8, Susan Dymecki9, Janan T Eppig10, Franziska B Grieder11, Nathaniel Heintz12, Geoff Hicks13, Thomas R Insel14, Alexandra Joyner15, Beverly H Koller16, K C Kent Lloyd17, Terry Magnuson18, Mark W Moore19, Andras Nagy20, Jonathan D Pollock21, Allen D Roses22, Arthur T Sands23, Brian Seed24, William C Skarnes25, Jay Snoddy26, Philippe Soriano27, David J Stewart28, Francis Stewart29, Bruce Stillman28, Harold Varmus30, Lyuba Varticovski31, Inder M Verma32, Thomas F Vogt33, Harald von Melchner34, Jan Witkowski35, Richard P Woychik36, Wolfgang Wurst37, George D Yancopoulos38, Stephen G Young39 & Brian Zambrowicz40

1  National Human Genome Research Institute, National Institutes of Health, Building 31, Room 4B09, 31 Center Drive, Bethesda, Maryland 20892, USA.

2  National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Building 31, Room 3C02, Bethesda, Maryland 20892, USA.

3  The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

4  Department of Genetics, University of Pennsylvania, 111 Clinical Research Building, 415 Curie Boulevard, Philadelphia, Pennsylvania 19104-6145, USA.

5  University of Utah, Eccles Institute of Human Genetics, Suite 5400, Salt Lake City, Utah 85112, USA.

6  National Human Genome Research Institute, National Institutes of Health, Building 31, Room 4B09, 31 Center Drive, Bethesda, Maryland 20892, USA.

7  McArdle Laboratory for Cancer Research, University of Wisconsin - Madison, 1400 University Avenue, Madison, Wisconsin 53706-1599, USA.

8  TPG Ventures, 345 California Street, Suite 2600, San Francisco, California 94104, USA.

9  Harvard Medical School, Department of Genetics, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA.

10  The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609-1500, USA.

11  National Center for Research Resources, National Institutes of Health, 1 Democracy Plaza, 6701 Democracy Boulevard, Bethesda, Maryland 20817-4874, USA.

12  Laboratory of Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.

13  Manitoba Institute of Cell Biology, 675 McDermot Avenue, Room ON5029, Winnipeg, Manitoba R3E 0V9, Canada.

14  National Institute of Mental Health, 6001 Executive Blvd. - Rm 8235- MSC 9669, Bethesda, Maryland 20892-9669, USA.

15  Skirball Institute of Biomolecular Medicine, 540 First Avenue, 4th Floor, New York, New York 10016, USA.

16  Department of Genetics, University of North Carolina, CB 7248, 7007 Thurston Bowles Bldg, Chapel Hill, North Carolina 27599, USA.

17  School of Veterinary Medicine, University of California, One Shields Avenue, Davis, California 95616, USA.

18  Department of Genetics, Room 4109D Neurosciences Research Building, University of North Carolina, CB 7264, 103 Mason Farm Road, Chapel Hill, North Carolina 27599, USA.

19  Deltagen, 740 Bay Road, Redwood City, California 94063-2469, USA.

20  Samuel Lumenfeld Research Institute, University of Toronto, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada.

21  National Institute on Drug Abuse, 6001 Executive Blvd, Rm 4274, Bethesda, Maryland 20892, USA.

22  GlaxoSmithKline, 5 Moore Drive, Durham, North Carolina 27709, USA.

23  Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, Texas 77381-1160, USA.

24  Department of Molecular Biology, Massachusetts General Hospital, Wellman 911, 55 Fruit Street, Boston, Massachusetts 02114, USA.

25  The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

26  The University of Tennessee-ORNL Graduate School of Genome Science and Technology, PO Box 2008, MS6164, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6164, USA.

27  Division of Basic Sciences, A2-025, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, P.O. Box 19024, Seattle, Washington 98109-1024, USA.

28  Cold Spring Harbor Laboratory, 1 Bungtown Road, PO Box 100, Cold Spring Harbor, New York 11724, USA.

29  Bioz, University of Technology, Dresden, c/o MPI-CBG, Pfotenhauerstr 108, 1307 Dresden, Germany.

30  Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA.

31  National Cancer Institute, National Institutes of Health, 31 Center Drive, Room 3A11, Bethesda, Maryland 20892-2440, USA.

32  Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037-1099, USA.

33  Merck Research Laboratories, PO Box 4, WP26-265, 770 Sumneytown Pike, West Point, Pennsylvania 19486, USA.

34  Laboratory for Molecular Hematology, University of Frankfurt Medical School, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany.

35  Banbury Center, Cold Spring Harbor Laboratory, PO Box 534, Cold Spring Harbor, New York 11724-0534, USA.

36  The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA.

37  Institute of Developmental Genetics, GSF Research Center, Max-Planck-Institute of Psychiatry, Ingolstaeder Landstr. 1, 85764 Munich/Neuherberg, Germany.

38  Regeneron Pharmaceuticals, 777 Old Saw Mill River Road, Tarrytown, New York 10591, USA.

39  Gladstone Foundation for Cardiovascular Disease, University of California, San Francisco, California, USA.

40  Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, Texas 77381-1160, USA.

Correspondence should be addressed to Christopher P Austin austinc@mail.nih.gov
Mouse knockout technology provides a powerful means of elucidating gene function in vivo, and a publicly available genome-wide collection of mouse knockouts would be significantly enabling for biomedical discovery. To date, published knockouts exist for only about 10% of mouse genes. Furthermore, many of these are limited in utility because they have not been made or phenotyped in standardized ways, and many are not freely available to researchers. It is time to harness new technologies and efficiencies of production to mount a high-throughput international effort to produce and phenotype knockouts for all mouse genes, and place these resources into the public domain.

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Nature Genetics
ISSN: 1061-4036
EISSN: 1546-1718
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