Abstract
Besides serving to package nuclear DNA, histones carry information in the form of a diverse array of post-translational modifications. Methylation of histones H3 and H4 has been implicated in long-term epigenetic 'memory'1. Dimethylation or trimethylation of Lys4 of histone H3 (H3 Lys4) has been found in expressible euchromatin of yeasts and mammals2,3,4. In contrast, methylation of Lys9 of histone H3 (H3 Lys9) has been implicated in establishing and maintaining the largely quiescent heterochromatin of mammals, yeasts, Drosophila melanogaster and plants5,6,7,8,9. We have previously shown that a DNA methylation mutant of Neurospora crassa, dim-5 (defective in methylation), has a nonsense mutation in the SET domain of an H3-specific histone methyltransferase and that substitutions of H3 Lys9 cause gross hypomethylation of DNA10. Similarly, the KRYPTONITE histone methyltransferase is required for full DNA methylation in Arabidopsis thaliana11. We used biochemical, genetic and immunological methods to investigate the specific mark for DNA methylation in N. crassa. Here we show that trimethylated H3 Lys9, but not dimethylated H3 Lys9, marks chromatin regions for cytosine methylation and that DIM-5 specifically creates this mark.
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Acknowledgements
We are grateful to M. Tachibana and Y. Shinkai for gifts of recombinant histone H3 tail plasmids, J.P. Jackson and S.E. Jacobsen for providing recombinant histone H3 tail proteins, G. Kothe for unpublished information on pcn, K. Noma for advice, former and present members of our laboratories for stimulating discussions and M. Freitag for comments on the manuscript. H.T. thanks L. van Beethoven for inspiration. This study was supported by grants from the US National Institutes of Health to E.U.S. and X.C.
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Tamaru, H., Zhang, X., McMillen, D. et al. Trimethylated lysine 9 of histone H3 is a mark for DNA methylation in Neurospora crassa. Nat Genet 34, 75–79 (2003). https://doi.org/10.1038/ng1143
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DOI: https://doi.org/10.1038/ng1143
