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Article
Nature Genetics  27, 48 - 54 (2001)
doi:10.1038/83751

Transcriptional regulation and function during the human cell cycle

Raymond J. Cho1, 6, 10, Mingxia Huang2, 10, Michael J. Campbell3, 7, 10, Helin Dong4, Lars Steinmetz1, Lisa Sapinoso8, Garret Hampton8, Stephen J. Elledge2, Ronald W. Davis1, 5 & David J. Lockhart4, 9

1  Department of Genetics, Stanford University School of Medicine, Stanford, California, USA.

2  Department of Biochemistry and Molecular Biology, Department of Molecular and Human Genetics, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas, USA.

3  Molecular Applications Group, Palo Alto, California, USA.

4  Affymetrix, Inc., Santa Clara, California, USA.

5  Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA.

6  Present address: InGenuity Systems, Inc., Alviso, California, USA.

7  Present address: Celera Genomics, Foster City, California, USA.

8  Genomics Institute of the Novartis Research Foundation, San Diego, California, USA.

9  Salk Institute for Biological Studies, Laboratory of Genetics, La Jolla, California, USA.

10  These authors contributed equally to this work.

Correspondence should be addressed to Michael J. Campbell michael.campbell@fc.celera.com
We report here the transcriptional profiling of the cell cycle on a genome-wide scale in human fibroblasts. We identified approximately 700 genes that display transcriptional fluctuation with a periodicity consistent with that of the cell cycle. Systematic analysis of these genes revealed functional organization within groups of coregulated transcripts. A diverse set of cytoskeletal reorganization genes exhibit cell-cycle−dependent regulation, indicating that biological pathways are redirected for the execution of cell division. Many genes involved in cell motility and remodeling of the extracellular matrix are expressed predominantly in M phase, indicating a mechanism for balancing proliferative and invasive cellular behavior. Transcripts upregulated during S phase displayed extensive overlap with genes induced by DNA damage; cell-cycle−regulated transcripts may therefore constitute coherent programs used in response to external stimuli. Our data also provide clues to biological function for hundreds of previously uncharacterized human genes.

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Nature Genetics
ISSN: 1061-4036
EISSN: 1546-1718
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