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Technical Report
Nature Neuroscience 9, 443 - 452 (2006)
Published online: 19 February 2006; | doi:10.1038/nn1654

FACS-array profiling of striatal projection neuron subtypes in juvenile and adult mouse brains

Mary Kay Lobo, Stanislav L Karsten, Michelle Gray, Daniel H Geschwind & X William Yang

Supplementary Fig. 1 (pdf 1M)
Analysis of cell death and stress related genes in sorted neurons.

Supplementary Fig. 2 (pdf 448K)
Gene Ontology analysis identifies enrichment in Signal Transduction (EASE score; p < 0.05).

Supplementary Fig. 3 (pdf 3M)
A subset of MSN-subtype-specific genes has striatal-enriched expression.

Supplementary Fig. 4 (pdf 3M)
Striatonigral neurons are more affected in the dorsal striatum compared to the ventral striatum (nuclear accumbens) in Ebf1-/- mice.

Supplementary Fig. 5 (pdf 2M)
Low amounts of starting RNA template yield reproducible and consistent results.

Supplementary Table 1 (pdf 56K)
Experimental chart of performed microarray hybridizations.

Supplementary Table 2 (pdf 68K)
qRT-PCR using Taqman Gene Expression Assays show enrichment of Evi3/Zfp521 and Ebf1 in the striatonigral MSNs.

Supplementary Table 3 (pdf 100K)
PCR primers used for RT-PCR validation of the striatal MSN-subtype specific genes and to make in situ hybridization probes.

Supplementary Methods (pdf 256K)


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Nature Neuroscience
ISSN: 1097-6256
EISSN: 1546-1726
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