Nature Neuroscience 9, 443 - 452 (2006)
Published online: 19 February 2006; | doi:10.1038/nn1654
FACS-array profiling of striatal projection neuron subtypes in juvenile and adult mouse brainsMary Kay Lobo, Stanislav L Karsten, Michelle Gray, Daniel H Geschwind
& X William Yang Supplementary Fig. 1 (pdf 1M) Analysis of cell death and stress related genes in sorted neurons. Supplementary Fig. 2 (pdf 448K) Gene Ontology analysis identifies enrichment in Signal Transduction (EASE score; p < 0.05). Supplementary Fig. 3 (pdf 3M) A subset of MSN-subtype-specific genes has striatal-enriched expression. Supplementary Fig. 4 (pdf 3M) Striatonigral neurons are more affected in the dorsal striatum compared to the ventral striatum (nuclear accumbens) in Ebf1-/- mice. Supplementary Fig. 5 (pdf 2M) Low amounts of starting RNA template yield reproducible and consistent results. Supplementary Table 1 (pdf 56K) Experimental chart of performed microarray hybridizations. Supplementary Table 2 (pdf 68K) qRT-PCR using Taqman Gene Expression Assays show enrichment of Evi3/Zfp521 and Ebf1 in the striatonigral MSNs. Supplementary Table 3 (pdf 100K) PCR primers used for RT-PCR validation of the striatal MSN-subtype specific genes and to make in situ hybridization probes. Supplementary Methods (pdf 256K)
|