Nature Neuroscience
8, 730 - 735 (2005)
Published online: 8 May 2005; | doi:10.1038/nn1459
XTRPC1-dependent chemotropic guidance of neuronal growth conesSangwoo Shim, Eyleen L Goh, Shaoyu Ge, Kurt Sailor, Joseph P Yuan, H Llewelyn Roderick, Martin D Bootman, Paul F Worley, Hongjun Song
& Guo-li MingSupplementary Fig. 1 (jpg 150K)
Neurite extension in the presence or absence of gradients of guidance cues. (a) Summary of neurite extension rates during the turning assay in a gradient of netrin-1 (5 g/ml in the pipette) for different neurons. Values represent mean  s.e.m. (n = 20-29). (b) Summary of neurite length of neurons derived from embryos injected with either the XTRPC1 morpholino or a control morpholino cultured without the addition of any guidance cues. Values represent mean s.e.m. (n = 25-27). No statistical significance was found (P > 0.05, Bootstrap test). Supplementary Fig. 2 (jpg 209K)
Density of commissural interneurons in the developing spinal cord of Xenopus embryos. (a-d) Sample projections of Z-stack confocal images of 3A10 staining (red) of Xenopus spinal cord (stage 30) from the sagittal views. The same segment of spinal cord was imaged from the side injected with the XTRPC1 morpholino and fixable FITC-dextran as a lineage tracer (green) as shown in (a) and from the uninjected side as shown in (c). Enlarged images of 3A10 staining were shown in (b, d). The cell bodies of commissural interneurons exhibit specific 3A10 staining, which is not as strong as those for axons. Note a reduction of 3A10+ fiber tracks in the un-injected side when compared to that in the injected side from these sagittal views. Scale bar: 100 m for (a,c) and 40 m for (b,d). (e) Quantification of the density of commissural interneurons in stage 30 embryos. The number of 3A10+ labelled cell bodies was counted from the uninjected and injected side within the same segment of the spinal cord of embryos unilaterally injected with the XTRPC1 morpholino at the two-cell stage. Values represent mean s.e.m. (n = 6). No statistical significance was found (P > 0.05, Bootstrap test) Supplementary Fig. 3 (jpg 145K)
A model of the involvement of TRPCs in cytoplasmic signalling of guidance cues. Supplementary Video 1 (mov 2M)
3D reconstruction of Z-series confocal images of the longitudinal axonal tracks of commissural interneurons in the ventral portion of the developing Xenopus spinal cord of stage 30 embryos injected with XTRPC1 morpholino. Shown are axons of commissural interneurons stained with monoclonal antibody 3A10 (red). The XTRPC1 morpholino was delivered to half of the embryo together with fixable FITC-dextran as a lineage tracer (green; see Methods). Supplementary Video 2 (mov 2M)
3D reconstruction of Z-series confocal images of the longitudinal axonal tracks of commissural interneurons in the ventral portion of the developing Xenopus spinal cord of stage 30 embryos injected with control morpholino. Shown are axons of commissural interneurons stained with monoclonal antibody 3A10 (red). Control morpholino was delivered to half of the embryo together with fixable FITC-dextran as a lineage tracer (green; see Methods).
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