Nature Neuroscience7, 1003 - 1009 (2004)
Published online: 26 August 2004; | doi:10.1038/nn1301
Differentiation of mouse embryonic stem cells into a defined neuronal lineage
Miriam Bibel1, 3, Jens Richter1, 3, Katrin Schrenk1, 3, Kerry Lee Tucker1, 3, Volker Staiger2, Martin Korte2, Magdalena Goetz2, 3
& Yves-Alain Barde1, 3
1
Friedrich Miescher Institute, CH-4058 Basel, Switzerland.
2
Max-Planck Institute of Neurobiology, 82152 Planegg-Martinsried, Germany.
3
Present addresses: Novartis Pharma AG Nervous System Research, 4002 Basel, Switzerland (M.B., J.R. and K.S.); University of Heidelberg, 69120 Heidelberg, Germany (K.L.T.); GSF Institute of Stem Cell Research, 85764 Neuherberg, Germany (M.G.); Biocenter, University of Basel, 4056 Basel, Switzerland (Y.-A.B.).
Although it has long been known that cultured embryonic stem cells can generate neurons, the lineage relationships with their immediate precursors remain unclear. We report here that selection of highly proliferative stem cells followed by treatment with retinoic acid generated essentially pure precursors that markers identified as Pax-6-positive radial glial cells. As they do in vivo, these cells went on to generate neurons with remarkably uniform biochemical and electrophysiological characteristics.
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