Nature Neuroscience
4, 781 - 782 (2001)
doi:10.1038/90476
CRMP-2 induces axons in cultured hippocampal neuronsNaoyuki Inagaki1, 2, Kazuyasu Chihara1, Nariko Arimura1, 3, Céline Ménager1, 3, Yoji Kawano1, 3, Naruhiro Matsuo1, Takashi Nishimura1, 3, Mutsuki Amano1, 3
& Kozo Kaibuchi1, 31
Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630-0101, Japan
2
Recognition and Formation, PRESTO, JST, Kumamoto 860-0012, Japan
3
Department of Cell Pharmacology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan
Correspondence should be addressed to Kozo Kaibuchi kaibuchi@med.nagoya-u.ac.jpIn cultured hippocampal neurons, one axon and several dendrites differentiate from a common immature process1. Here we found that CRMP-2/TOAD-64/Ulip2/DRP-2 (refs. 2−4) level was higher in growing axons of cultured hippocampal neurons, that overexpression of CRMP-2 in the cells led to the formation of supernumerary axons and that expression of truncated CRMP-2 mutants suppressed the formation of primary axon in a dominant-negative manner. Thus, CRMP-2 seems to be critical in axon induction in hippocampal neurons, thereby establishing and maintaining neuronal polarity.
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