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CRMP-2 induces axons in cultured hippocampal neurons

Nature Neuroscience volume 4, pages 781–782 (2001)Cite this article

Abstract

In cultured hippocampal neurons, one axon and several dendrites differentiate from a common immature process1. Here we found that CRMP-2/TOAD-64/Ulip2/DRP-2 (refs. 2–4) level was higher in growing axons of cultured hippocampal neurons, that overexpression of CRMP-2 in the cells led to the formation of supernumerary axons and that expression of truncated CRMP-2 mutants suppressed the formation of primary axon in a dominant-negative manner. Thus, CRMP-2 seems to be critical in axon induction in hippocampal neurons, thereby establishing and maintaining neuronal polarity.

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Figure 1: Higher levels of CRMP-2 in the growing axons of cultured hippocampal neurons.
Figure 2: Overexpression of CRMP-2 in cultured hippocampal neurons induces multiple axon formation.
Figure 3: Effects of EGFP-CRMP-2 and CRMP-2 mutants on the morphologies of cultured hippocampal neurons.

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Acknowledgements

We thank Y. Gu and Y. Ihara for providing monoclonal anti-CRMP-2 antibody C4G, J. Yamada and A. Takemura for technical assistance, and W.J. Nelson, M. Nakafuku, Y. Goshima, H. Qadota, C.G. Dotti and Y. Fukata for discussions. This research was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan, the Japan Society for the Promotion of Science Research for the Future, the Human Frontier Science Program and Uehara Memorial Foundation.

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Authors and Affiliations

  1. Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma, 630-0101, Japan

    Naoyuki Inagaki, Kazuyasu Chihara, Nariko Arimura, Céline Ménager, Yoji Kawano, Naruhiro Matsuo, Takashi Nishimura, Mutsuki Amano & Kozo Kaibuchi

  2. Recognition and Formation, PRESTO, JST, Kumamoto, 860-0012, Japan

    Naoyuki Inagaki

  3. Department of Cell Pharmacology, Nagoya University Graduate School of Medicine, Nagoya, 466-8550, Japan

    Nariko Arimura, Céline Ménager, Yoji Kawano, Takashi Nishimura, Mutsuki Amano & Kozo Kaibuchi

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  1. Naoyuki Inagaki
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Correspondence to Kozo Kaibuchi.

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Inagaki, N., Chihara, K., Arimura, N. et al. CRMP-2 induces axons in cultured hippocampal neurons. Nat Neurosci 4, 781–782 (2001). https://doi.org/10.1038/90476

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