Articles in 2019

The loss of homologous recombination (HR) genes such as BRCA1 and BRCA2 is deleterious to the survival of normal cells, yet it is tolerated in cancer cells. Here the authors identify TNFα signaling as a determinant of viability in BRCA2- inactivated cancer cells.

Single-cell RNA sequencing allows characterizing cell-to-cell heterogeneity at transcriptome scale. Here, the authors present an approach that enables microRNA and mRNA sequencing in the same single cell, providing insights into the origins of non-genetic cellular variability and mechanisms of miRNA regulation.

Urolithins are microbial metabolites derived from food polyphenols. Here, Singh et al. show that urolithin A and a synthetic analogue enhance gut barrier function via Nrf2-dependent pathways and mitigate inflammation and colitis in mice, highlighting a potential application for inflammatory bowel diseases.

Changes in S-isotope ratios over time provide clues to understanding the co-evolution of Earth and its biosphere. Here the authors determine the isotope effect of the first reductive enzyme in the sulfate respiration pathway and reinterpret sedimentary S-isotope records based on this biochemical constraint.

In considering cross-feeding among microbes within communities, it is typically assumed that metabolic secretions are costly to produce. However, Pacheco et al. use metabolic models to show that ‘costless’ secretions could be common in some environments and important for structuring interactions among microbes.

In contrast to steels, fusion welding high strength aluminum alloys such as AA7075 is notoriously difficult. Here, the authors add nanoparticles to a weld filler rod to successfully weld AA7075 without hot cracks or loss of strength at the weld.

The human genome includes a large amount of repetitive sequence, such as human satellite II (HSATII), but their function remains largely unknown. Here, Nogalski et al. show that herpesvirus infection induces HSATII RNA expression, which in turn affects virus replication and cell motility.

Most tumours are characterized by increased aerobic glycolytic activity. Here the authors show that elevated  aerobic glycolysis is not essential for cancer initiation by testing the effect of lactate dehydrogenase depletion on the ability of hair follicle stem cells (HFSCs) to form squamous cell carcinoma (SCC) in mouse genetic models.

Squalene epoxidase (SQLE) is a key enzyme in cholesterol biosynthesis and is a target for hypercholesteremia and cancer drug development. Here the authors present the crystal structures of the human SQLE catalytic domain alone and bound with small molecule inhibitors, which will facilitate the development of next-generation SQLE inhibitors.

The demethylase activity of KDM5A is allosterically enhanced by binding of histone H3 to its PHD1 reader domain, through an unknown mechanism. Here the authors show that the PHD1 domain drives ligand-induced allosteric stimulation by stabilizing the binding of substrate to the catalytic domain.

Reducing particle size of electrode materials to nanoscale dimensions is believed responsible for their enhanced reaction kinetics and electrochemical performance. Here, the authors use in situ transmission electron microscopy to study the dynamic process of the spinel zinc ferrite nanoparticles as a function of size, finding that the intercalation reaction pathway changes below a critical particle size.

Cancer cells are metabolically adaptable and the identification of specific vulnerabilities is challenging. Here the authors identify a subset of neuroendocrine cell lines exquisitely sensitive to inhibition of SQLE, an enzyme in the cholesterol biosynthetic pathway, due to the toxic accumulation of pathway intermediate squalene.

A planetary-scale streak structure in the Venusian lower-cloud layer was observed by the Akatsuki orbiter. Here the authors present numerical simulations that reproduce such structure and reveal that a low-stability layer, which induces baroclinic disturbances, is essential for its formation.

Measuring multiple neurophysiologic variables usually requires bulky benchtop optical systems and working with anesthetized animals. Here the authors present a miniature portable microscope for neurovascular imaging in awake rodents, combining fluorescence, intrinsic optical signals and laser speckle contrast.

Biological light-driven ion pumps move ions against a concentration gradient to create a membrane potential, converting sunlight into an osmotic potential. Here, the authors make an artificial ion pump which drives ions thermodynamically uphill against a large concentration gradient upon illumination, which can be used for harvesting solar energy.

Traditional water–gas shift reaction process is hindered by harsh reaction conditions and extra steps for hydrogen separation and purification. Here, the authors report a room temperature electrochemical water–gas shift process for direct production of high purity hydrogen with a faradaic efficiency of approximately 100%.

Recent research efforts have aimed at discovering thermoelectric materials with high efficiency in the middle-low temperature range, where a majority of waste heat is lost to the ambient. Here, the authors discover colossal Seebeck coefficient values in metallic copper selenide from 340 K to 400 K.

Sterile Insect Technique (SIT) is used to suppress wild populations. Here the authors integrate CRISPR-based technology and SIT to develop a precision guided SIT (pgSIT), and demonstrate its proof-of-principle by generating 100% sterile males.

Examples of overdominance are usually explained by deleterious effects in homozygotes. Here, Kellenberger et al. describe a case of overdominance in the floral color of the Alpine orchid Gymnadenia rhellicani apparently maintained by pollinator preferences without deleterious effects in homozygotes.

Mutations in the ATM tumor suppressor gene confer hypersensitivity to DNA-damaging chemotherapeutic agents. Here, the authors provide evidence that these hypersensitivities reflect a crucial role for ATM at damaged replication forks being to prevent toxic DNA end-joining leading to chromosome fusions and cell death.