Abstract
The secreted peptide gene CLAVATA3 (CLV3) regulates stem cell fate in the shoot apical meristem in Arabidopsis thaliana plants, but the molecular structure of the active mature CLV3 peptide is controversial. Here, using nano-LC-MS/MS analysis of apoplastic peptides of A. thaliana plants overexpressing CLV3, we show that CLV3 is a 13-amino-acid arabinosylated glycopeptide. Post-translational arabinosylation of CLV3 is critical for its biological activity and high-affinity binding to its receptor CLV1.
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Acknowledgements
This research was supported by a Grant-in-Aid for Scientific Research for Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) (No. 19060010), a Grant-in-Aid for Young Scientists from MEXT (No. 18687003), a Grant-in-Aid for Creative Scientific Research from the Japan Society for the Promotion of Science (No. 19GS0315) and the Program of Basic Research Activities for Innovative Biosciences from the Bio-oriented Technology Research Advancement Institution (BRAIN). We thank the Radioisotope Research Center of Nagoya University for instrumental support in radioisotope experiments.
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K.O., H.S. and M.O.-O. performed and analyzed experiments. K.O. identified glycopeptides and performed bioassays. H.S. performed sugar linkage analysis and receptor binding assays. M.O.-O. generated transgenic plants. Y.M. designed, performed and analyzed experiments and prepared the manuscript.
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Ohyama, K., Shinohara, H., Ogawa-Ohnishi, M. et al. A glycopeptide regulating stem cell fate in Arabidopsis thaliana. Nat Chem Biol 5, 578–580 (2009). https://doi.org/10.1038/nchembio.182
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DOI: https://doi.org/10.1038/nchembio.182
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