The NMR structures of three intermediates bound to the pyridoxal-synthesizing enzyme Pdx1 illuminate its complex catalytic cycle. The success of this approach with a high-molecular-weight protein should encourage further applications of NMR techniques for characterizing enzyme reaction pathways.
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Townsend, C. Peering inside the black box to find enzyme-bound intermediates. Nat Chem Biol 4, 390–391 (2008). https://doi.org/10.1038/nchembio0708-390
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DOI: https://doi.org/10.1038/nchembio0708-390
