New electrophilic reagents for activity-based protein profiling (ABPP) can discriminate between nucleophilic amino acids, such as cysteine, that are in different protein microenvironments. These reagents extend the reach of ABPP beyond serine proteases and other proteins with hyperreactive nucleophiles.
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Kodadek, T. Proteomes take the electrophilic bait. Nat Chem Biol 4, 387–388 (2008). https://doi.org/10.1038/nchembio0708-387
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DOI: https://doi.org/10.1038/nchembio0708-387