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Do-it-yourself enzymes

Nature Chemical Biology volume 4, pages 273–275 (2008)Cite this article

Enzymes have long captivated biologists and chemists by performing difficult reactions rapidly and selectively in aqueous environments. The designs of enzymes catalyzing a retro-aldol reaction and a Kemp elimination demonstrate that complex, biochemically novel reactions can be achieved using cutting-edge computational methods.

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Figure 1: The enzyme assembly line.

References

  1. Harbury, P.B., Plecs, J.J., Tidor, B., Alber, T. & Kim, P.S. Science 282, 1462–1467 (1998).

    Article  CAS  Google Scholar 

  2. Kuhlman, B. et al. Science 302, 1364–1368 (2003).

    Article  CAS  Google Scholar 

  3. Looger, L.L., Dwyer, M.A., Smith, J.J. & Hellinga, H.W. Nature 423, 185–190 (2003).

    Article  CAS  Google Scholar 

  4. Yin, H. et al. Science 315, 1817–1822 (2007).

    Article  CAS  Google Scholar 

  5. Bolon, D.N. & Mayo, S.L. Proc. Natl. Acad. Sci. USA 98, 14274–14279 (2001).

    Article  CAS  Google Scholar 

  6. Kaplan, J. & DeGrado, W.F. Proc. Natl. Acad. Sci. USA 101, 11566–11570 (2004).

    Article  CAS  Google Scholar 

  7. Jiang, L. et al. Science 319, 1387–1391 (2008).

    Article  CAS  Google Scholar 

  8. Rothlisberger, D. et al. Nature advance online publication, 10.1038/nature06879 (26 March 2008).

  9. Hilvert, D. Annu. Rev. Biochem. 69, 751–792 (2000).

    Article  CAS  Google Scholar 

  10. Tantillo, D.J., Chen, J. & Houk, K.N. Curr. Opin. Chem. Biol. 2, 743–750 (1998).

    Article  CAS  Google Scholar 

  11. Zanghellini, A. et al. Protein Sci. 15, 2785–2794 (2006).

    Article  CAS  Google Scholar 

  12. Ladman, Y. et al. IEEE Trans. Robot. Autom. 6, 578–589 (1990).

    Article  Google Scholar 

  13. Walsh, C. Nature 409, 226–231 (2001).

    Article  CAS  Google Scholar 

  14. Chakrabarti, R., Klibanov, A.M. & Friesner, R.A. Proc. Natl. Acad. Sci. USA 102, 12035–12040 (2005).

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Vikas Nanda is at the Center for Advanced Biotechnology and Medicine and the Department of Biochemistry at the Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 679 Hoes Lane West, Piscataway, New Jersey 08854, USA. nanda@cabm.rutgers.edu,

    Vikas Nanda

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  1. Vikas Nanda
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Nanda, V. Do-it-yourself enzymes. Nat Chem Biol 4, 273–275 (2008). https://doi.org/10.1038/nchembio0508-273

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