Brief Communication abstract
Nature Chemical Biology 3, 650 - 651 (2007)
Published online: 23 August 2007 | doi:10.1038/nchembio.2007.30
Light-controlled gene silencing in zebrafish embryos
Ilya A Shestopalov1,3, Surajit Sinha1,2,3 & James K Chen1
Functional genomic studies in zebrafish frequently use synthetic oligonucleotides called morpholinos that block RNA splicing or translation. However, the constitutive activity of these reagents limits their experimental utility. We report here the synthesis of a photoactivatable morpholino targeting the no tail (ntl) gene. This caged reagent permits spatiotemporal gene regulation in vivo and the photochemical generation of functionally mosaic organisms.
- Department of Chemical and Systems Biology, Stanford University School of Medicine, 269 Campus Drive, CCSR 3155, Stanford, California 94305, USA.
- Present address: Department of Organic Chemistry, Indian Association for the Cultivation of Science, 2A and 2B Raja S.C. Mullick Road, Jadavpur, Kolkata 700032, India.
- These authors contributed equally to this work.
Correspondence to: James K Chen1 e-mail: jameschen@stanford.edu
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