The goal of high-throughput screening (HTS) from the perspective of the biologist is to identify a highly specific small molecule that can be used to inhibit a protein in its normal biological context. Although several useful small molecules have been identified with HTS, there are many challenges to be considered when contemplating a screen, especially by those unfamiliar with chemical biology.
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The authors thank D. Halpin and J. Williams for useful discussions.
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Soderholm, J., Uehara-Bingen, M., Weis, K. et al. Challenges facing the biologist doing chemical genetics. Nat Chem Biol 2, 55–58 (2006). https://doi.org/10.1038/nchembio0206-55
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DOI: https://doi.org/10.1038/nchembio0206-55