Activity-based probes can be used for monitoring enzyme activity based on their covalent reactions with active-site residues. A quenched activity-based probe has now been developed that becomes fluorescent only after labeling active proteases. The specificity of the fluorescent signal and cell permeability of the small molecule make this probe effective for monitoring protease activity in living cells.
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Brix, K., Jordans, S. Watching proteases in action. Nat Chem Biol 1, 186–187 (2005). https://doi.org/10.1038/nchembio0905-186
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DOI: https://doi.org/10.1038/nchembio0905-186
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