Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

How FDA Approved Chymosin: A Case History

Bio/Technology volume 9, pages 349–351 (1991)Cite this article

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Foltmann, B. 1970. Prochymosin and Chymosin (Prorennin and Rennin). Methods in Enzymology 19: 421.

    Article  Google Scholar 

  2. Burgess, K. and Shaw, M. 1983. Dairy, pp. 260–265 in Industrial Enzymology, T. Godfrey and J. Reichelt (eds.), The Nature Press, New York.

    Google Scholar 

  3. Foltmann, B. et al. 1979. The Primary Structure of Calf Chymosin. J. Biol. Chem. 254: 8447.

    CAS  PubMed  Google Scholar 

  4. Hidaka, M. et al. 1986. Cloning and Structural Analysis of the Calf Prochymosin Gene. Gene 43: 197.

    Article  CAS  Google Scholar 

  5. Pederson, V.B. and Foltmann, B. 1975. Amino Acid Sequence of the Peptide Segment Liberated During Activation of Prochymosin. Eur. J. Biochem. 55: 95.

    Article  Google Scholar 

  6. Harris, T.J.R. et al. 1982. Molecular Cloning and Nucleotide Sequence of cDNA Coding for Calf Preprochymosin. Nucleic Acids Research 10: 2177.

    Article  CAS  Google Scholar 

  7. Moir, D. et al. 1982. Molecular Cloning and Characterization of Double-stranded Complementary DNA Coding for Bovine Chymosin EC 3.4.23.4. Gene 19: 127

    Article  CAS  Google Scholar 

  8. Emtage, J.S. et al. l983. Synthesis of Calf Prochymosin (Prorennin) in Escherichia coli. PNAS 80: 3671.

    Article  Google Scholar 

  9. McCaman, M.T., Andrews, W.H. and Files, J.G. 1985. Enzymatic Properties and Processing of Bovine Prochymosin synthesized in Escherichia coli. Journal of Biotechnology 2: 177.

    Article  CAS  Google Scholar 

  10. Goff, C.G. et al. 1984. Expression of Calf Prochymosin in Saccharomyces cerevisiae. Gene 27: 35.

    Article  CAS  Google Scholar 

  11. Cullen, D. et al. 1987. Controlled Expression and Secretion of Bovine Chymosin in Aspergillus nidulans. Bio/Technology 5: 369.

    CAS  Google Scholar 

  12. Mellor, J. et al. 1983. Efficient Synthesis of Enzymatically Active Calf Chymosin in Saccharomyces cerevisiae. Gene 24: 1.

    Article  CAS  Google Scholar 

  13. Kawaguchi, Y. et al. 1987. Production of Chymosin in Escherichia coli Cells and its Enzymatic Properties. Agricultural and Biological Chemistry 51: 1871.

    CAS  Google Scholar 

  14. Marston, F.A.O. et al. 1984. Purification of Calf Prochymosin (Prorennin) in Escherichia coli. Bio/Technology 2: 800.

    CAS  Google Scholar 

  15. Gorbach, S.L. 1978. Recombinant DNA: An Infectious Disease Perspective. Journal of Infectious Diseases 137: 615.

    Article  CAS  Google Scholar 

  16. Curtiss, R. 1978. Biological Containment and Cloning Vector Transmissibility. Journal of Infectious Diseases. 137: 668.

    Article  CAS  Google Scholar 

  17. Smith, H.W. 1978. Is It Safe to Use Escherichia coli K-12 in Recombinant DNA Experiments?” Journal of Infectious Diseases 137: 655.

    Article  CAS  Google Scholar 

  18. Newland, J.W. and Neill, R.J. 1988. DNA Probes for Shiga-Like Toxins I and II and for Toxin-Converting Bacteriophages. J. Clin. Micr. 26: 1292.

    CAS  Google Scholar 

  19. Sutcliffe, J.G. 1978. Nucleotide Sequence of the Ampicillin Resistance Gene of E. coli Plasmid pBR322. PNAS 75: 3737.

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Eric L. Flamm: The opinions in this paper are the author's own and do not necessarily reflect those of the Food and Drug Administration.

Authors and Affiliations

  1. with the Office of Biotechnology, U.S. Food and Drug Administration, HF-6, 5600 fishers Lane, Rockville, MD, 20857

    Eric L. Flamm

Authors
  1. Eric L. Flamm
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Flamm, E. How FDA Approved Chymosin: A Case History. Nat Biotechnol 9, 349–351 (1991). https://doi.org/10.1038/nbt0491-349

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt0491-349

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing