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Improvement of a Baculovirus Pesticide by Deletion of the EGT Gene

Bio/Technology volume 9, pages 1086–1089 (1991)Cite this article

Abstract

The egt gene of the insect baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) encodes the enzyme ecdysteroid UDP–glucosyltransferase. Expression of egt enables the virus to inhibit molting of its infected host. In this study, we have compared the growth and development of insects infected by viruses with and without a functional egt gene. Our data reveal that, since insects normally stop feeding for both larval–larval and larval–pupal molts, expression of egt prevents the insect from experiencing this feeding arrest. Thus, egt functions to prolong the length of time the insect feeds after infection, with a resultant increase in the weight gain of the insect. We show that deletion of the egt gene significantly improves the pesticide characteristics of AcMNPV. Larvae infected with an egt deletion mutant display considerably reduced feeding and earlier mortality than wild–type AcMNPV–infected larvae.

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References

  1. Entwistle, P.D. and Evans, H.F. 1985. Viral control, p. 347–412. In: Comprehensive Insect Physiology, Biochemistry and Pharmacology, Vol. 12. Gilbert, L. I., and Kerkut, G. A. (Eds.). Pergamon Press, Oxford.

    Google Scholar 

  2. Huber, J. 1986. Use of baculoviruses in pest management programs, p. 181–202. In: The Biology of Baculoviruses, Vol. 2. Granados, R. R., and Federici, B. A. (Eds.). CRC Press, Boca Raton, FL.

    Google Scholar 

  3. Gröner, A. 1986. Specificity and safety of baculoviruses, p. 177–202. In: The Biology of Baculoviruses, Vol. 1. Granados, R. R., and Federici, B. A. (Eds.). CRC Press, Boca Raton, FL.

    Google Scholar 

  4. Carbonell, L.F., Klowden, M.J. and Miller, L.K. 1985. Baculovirus-mediated expression of bacterial genes in dipteran and mammalian cells. J. Virol. 56: 153–160.

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Carbonell, L.F. and Miller, L.K. 1987. Baculovirus interaction with non-target organisms: a virus-borne reporter gene is not expressed in two mammalian cell lines. Appl. Environ. Microbiol. 53: 1412–1417.

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Summers, M.D., Engler, R., Falcon, L.A. and Vail, P.V. (Eds.). 1975. Baculoviruses for Insect Pest Control: Safety Considerations. American Society for Microbiology, Washington, D.C.

    Google Scholar 

  7. Miller, L.K., Lingg, A.J. and Bulla, L.A., 1983. Bacterial, viral and fungal insecticides. Science 219: 715–721.

    Article  CAS  PubMed  Google Scholar 

  8. Carbonell, L.F., Hodge, M.R., Tomalski, M.D. and Miller, L.K. 1988. Synthesis of a gene coding for an insect-specific scorpion neurotoxin and attempts to express it using baculovirus vectors. Gene 73: 409–418.

    Article  CAS  PubMed  Google Scholar 

  9. Keeley, L.L. and Hayes, T.K. 1987. Speculations on biotechnology applications for insect neuroendocrine research. Insect Biochem. 17: 639–651.

    Article  CAS  Google Scholar 

  10. Martens, J.W.M., Honee, G., Zuidema, D., van Lent, J.W.M., Visser, B. and Vlak, J.M. 1990. Insecticidal activity of a bacterial crystal protein expressed by a recombinant baculovirus in insect cells. Appl. Environ. Microbiol. 56: 2764–2770.

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Merryweather, A.T., Weyer, U., Harris, M.P.G., Hirst, M., Booth, T. and Possee, R.D. 1990. Construction of genetically engineered baculovirus insecticides containing the Bacillus thuringensis subsp. kurstaki HD-73 delta endotoxin. J. Gen. Virol. 71: 1535–1544.

    Article  CAS  PubMed  Google Scholar 

  12. Tomalski, M.D. and Miller, L.K. 1991. Insect paralysis by baculovirus-mediated expression of a mite neurotoxin gene. Nature 352: 82–85.

    Article  CAS  PubMed  Google Scholar 

  13. Maeda, S. 1989. Increased insecticidal effect by a recombinant baculovirus carrying a synthetic diuretic hormone gene. Biochem. Biophys. Res. Comm. 165: 1177–1183.

    Article  CAS  PubMed  Google Scholar 

  14. Eldridge, R., Horodyski, F.M., Morion, D.B., O'Reilly, D.R., Truman, J.W., Riddiford, L.M. and Miller, L.K. 1991. Expression of an eclosion hormone gene in insect cells using baculovirus vectors. Insect Biochem. 21: 341–351.

    Article  CAS  Google Scholar 

  15. Hammock, B.D., Bonning, B., Possee, R.D., Hanzlik, T.N. and Maeda, S. 1990. Expression and effects of the juvenile hormone esterase in a baculovirus vector. Nature 344: 458–461.

    Article  CAS  Google Scholar 

  16. O'Reilly, D.R. and Miller, L.K. 1989. A baculovirus blocks insect molting by producing ecdysteroid UDP-glucosyltransferase. Science 245: 1110–1112.

    Article  CAS  PubMed  Google Scholar 

  17. O'Reilly, D.R. and Miller, L.K. 1990. Regulation of expression of a baculovirus ecdysteroid UDP-glucosyltransferase gene. J. Virol. 64: 1321–1328.

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Riddiford, L.M. 1985. Hormone action at the cellular level, p. 37–48. In: Comprehensive Insect Physiology, Biochemistry and Pharmacology, Vol. 8. Gilbert, L. I. and Kerkut, G. A. (Eds.). Pergamon Press, Oxford.

    Google Scholar 

  19. Smith, S.L. 1985. Regulation of ecdysteroid titer: synthesis, p. 295–341. In: Comprehensive Insect Physiology, Biochemistry and Pharmacology, Vol. 7. Gilbert, L. I. and Kerkut, G. A. (Eds.). Pergamon Press, Oxford.

    Google Scholar 

  20. Koolman, J. and Karlson, P. 1985. Regulation of ecdysteroid tiler: degradation, p. 343–361. In: Comprehensive Insecl Physiology, Biochemistry and Pharmacology, Vol. 7. Gilbert, L. I. and Kerkut, G. A. (Eds.). Pergamon Press, Oxford.

    Google Scholar 

  21. O'Reilly, D.R., Howarth, O.W., Rees, H.H. and Miller, L.K. 1991. Structure of the ecdysone glucoside formed by a baculovirus ecdysteroid UDP-glucosyltransferase. Insect Biochem. In press.

    Google Scholar 

  22. Subrahmanyam, B. and Ramakrishnan, N. 1981. Influence of a baculovirus infection on molting and food consumption by Spodoptera litura. J. Invertbr. Pathol. 38: 161–168.

    Article  Google Scholar 

  23. Lee, H.H. and Miller, L.K. 1978. Isolation of genotypic variants of Autographa californica nuclear polyhedrosis virus. J. Virol. 27: 754–767.

    CAS  PubMed  PubMed Central  Google Scholar 

  24. Miller, D.W., Safer, P. and Miller, L.K. 1986. An insecl baculovirus host-vector system for high level expression of foreign genes, p. 277–298. In: Genetic Engineering, Vol. 8. Setlow, J. K. and Hollander, A. (Eds.). Plenum Press, N.Y.

    Chapter  Google Scholar 

  25. Burton, R.L. 1969. Mass rearing the corn earworm in the laboratory. USDA-ARS (Series) 33–134.

  26. Miller, L.K. and Dawes, K.P. 1978. Restriction endonuclease analysis for the identification of baculovirus pesticides. Appl. Environ. Microbiol. 35: 411–421.

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Daum, R.J. 1970. A revision of two computer programs for probit analysis. Bull. Entomol. Soc. America 16: 10–15.

    Article  Google Scholar 

  28. Bliss, C.I. 1937. The calculation of the time-mortality curve. Ann. Appl. Biol. 24: 815–852.

    Article  CAS  Google Scholar 

  29. Litchfield, J.T. 1949. A method for rapid graphic solution of time-percent effect curves.J. Pharmacol. Exp. Ther. 97: 399–408.

    PubMed  Google Scholar 

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  1. David R. O'Reilly: Corresponding author.

Authors and Affiliations

  1. Departments of Entomology and Genetics, University of Georgia, Athens, Georgia, 30602

    David R. O'Reilly & Lois K. Miller

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  1. David R. O'Reilly
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  2. Lois K. Miller
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O'Reilly, D., Miller, L. Improvement of a Baculovirus Pesticide by Deletion of the EGT Gene. Nat Biotechnol 9, 1086–1089 (1991). https://doi.org/10.1038/nbt1191-1086

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