Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Research Paper
  • Published:

Genetically Transformed Cotton (Gossypium Hirsutum L.) Plants

Bio/Technology volume 5, pages 263–266 (1987)Cite this article

Abstract

Genetically engineered plants of commercial cotton varieties were obtained by Agrobacterium–mediated transformation. Inoculated tissues selected on kanamycin–containing medium gave rise to transformed calli that were resistant to the antibiotic and expressed the neomycin phosphotransferase enzyme. Amplified tissues were plated onto hormone–free medium to promote embryogenesis. Somatic embryos germinated and whole plants also expressed the marker enzyme.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Fraley, R., Rogers, S. and Horsch, R. 1986. Genetic transformation in higher plants. CRC Critical Reviews in Plant Science 4: 1–46.

    Article  CAS  Google Scholar 

  2. Abel, P., Nelson, R., De, B., Hoffmann, N., Rogers, S., Fraley, R. and Beachy, R. 1986. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232: 738–743.

    Article  CAS  Google Scholar 

  3. Comai, L., Facciotti, D., Hiatt, W., Thompson, G., Rose, R. and Stalker, D. 1985. Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317: 741–744.

    Article  CAS  Google Scholar 

  4. Shah, D., Horsch, R., Klee, H., Kishore, G., Winter, J., Tumer, N., Hironaka, C., Sanders, P., Gasser, C., Aykent, S., Siegel, N., Rogers, S. and Fraley, R. 1986. Engineering herbicide tolerance in transgenic plants. Science 233: 478–481.

    Article  CAS  Google Scholar 

  5. Fraley, R., Rogers, S., Horsch, R., Sanders, P., Flick, J., Adams, S., Bittner, M., Brand, L., Fink, C., Fry, J., Galluppi, G., Goldberg, S., Hoffmann, N. and Woo, S. 1983. Expression of bacterial genes in plant cells. Proc. Natl. Acad. Sci. USA 80: 4803–4807.

    Article  CAS  Google Scholar 

  6. McCormick, S., Niedermeyer, J., Fry, J., Barnason, A., Horsch, R. and Fraley, R. 1986. Lead disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens. Plant Cell Rep. 5: 81–84.

    Article  CAS  Google Scholar 

  7. Brisson, M., Paszkowski, J., Penswick, J., Gronenborn, B., Potrykus, I. and Hohn, T. 1984. Expression of a bacterial gene in plants by using a viral vector. Nature 310: 511–514.

    Article  CAS  Google Scholar 

  8. Shahin, E. and Simpson, R. 1986. Gene transfer system for potato. Hort Science 21: 1199–1201.

    CAS  Google Scholar 

  9. Fromm, M., Taylor, L. and Walbot, V. 1986. Stable transformation of maize after gene transfer by electroporation. Nature 319: 791–793.

    Article  CAS  Google Scholar 

  10. Potrykus, I., Saul, M., Petruska, J., Paszkowski, J. and Shillito, R. 1985. Direct gene transfer to cells of a graminaceous monocot. Mol. Gen. Genet. 199: 183–188.

    Article  CAS  Google Scholar 

  11. Zhou, G.-Y., Weng, J., Zeng, Y., Huang, J., Qian, S. and Liu, G. 1983. Introduction of exogenous DNA into cotton embryos. Meth. Enzymol. 101: 433–481.

    Article  CAS  Google Scholar 

  12. Stewart, J. and Hsu, C. 1977. In-ovulo embryo culture and seedling development of cotton (Gossypium hirsutum L.). Planta 137: 113–117.

    Article  CAS  Google Scholar 

  13. Peerbolte, R. 1986. The fate of T-DNA during vegetative and generative propagation—crown gall and hairy root tissues of Nicotiana spp. Ph.D. diss. State Univ. of Leiden, Leiden, The Netherlands.

  14. Jones, J., Dunsmuir, P. and Bedbrook, J. 1985. High level expression of introduced chimaeric genes in regenerated transformed plants. EMBO J. 4: 2411–2418

    Article  CAS  Google Scholar 

  15. deFramond, A., Barton, K. and Chilton, M.-D. 1983. Mini-Ti: A new vector strategy for plant genetic engineering. Bio/Technology 1: 262–269.

    Google Scholar 

  16. An, G., Watson, B., Stachel, S., Gordon, M. and Nester, E. 1985. New cloning vehicles for transformation of higher plants. EMBO J. 4: 277–284.

    Article  CAS  Google Scholar 

  17. Klee, H., Yanofsky, M. and Nester, E. 1985. Vectors for transformation of higher plants. Bio/Technology 3: 637–642.

    CAS  Google Scholar 

  18. Bevan, M. 1984. Binary Agrobacterium vectors for plant transformation. Nuc. Acids Res. 12: 8711–8721.

    Article  CAS  Google Scholar 

  19. Gorman, C., Moffat, L. and Howard, B. 1982. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol. 2: 1044–1051.

    Article  CAS  Google Scholar 

  20. Depicker, A., Stachel, S., Dhases, P., Zambryski, P. and Goodman, H. 1982. Nopaline synthase: Transcript mapping and DNA sequence. J. Mol. Appl. Genet. 1: 561–573.

    CAS  Google Scholar 

  21. Beck, E., Ludwig, G., Auerswald, E., Reiss, B. and Schaller, H. 1982. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene 19: 327–336.

    Article  CAS  Google Scholar 

  22. Rodgers, S., Fraley, R., Horsch, R., Levine, A., Flick, J., Brand, L., Fink, C., Moyer, T., O'Connell, K. and Sanders, R. 1985. Evidence for ribosome scanning during translation initiation of RNAs in transformed plant cell. Plant Mol. Biol. Rep. 3: 111–116.

    Article  Google Scholar 

  23. Hoekema, A., Hirsch, P., Hooykaas, P. and Schilperoort, R. 1983. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303: 179–180.

    Article  CAS  Google Scholar 

  24. Ditta, G., Stanfield, S., Corkin, D. and Helinski, D. 1980. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci. USA 77: 7347–7351.

    Article  CAS  Google Scholar 

  25. Miller, J. 1972. Experiments in Molecular Genetics, p. 431. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

    Google Scholar 

  26. Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.

    Article  CAS  Google Scholar 

  27. Gamborg, O., Miller, R. and Ojima, K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158.

    Article  CAS  Google Scholar 

  28. Trolinder, N. and Goodin, J. 1986. Somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.). Plant Cell Rep. In Press.

  29. Reiss, B., Sprengel, R., Will, H. and Schaller, H. 1984. A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extracts. Gene 30: 211–218.

    Article  CAS  Google Scholar 

  30. Herrera-Estrella, L., Depicker, A., Van Montagu, M. and Schell, J. 1983. Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector. Nature 303: 209–213.

    Article  CAS  Google Scholar 

  31. Taylor, B. and Powell, A. 1983. Isolation of plant DNA and RNA. B.R.L. Focus 4: 4–6.

    Google Scholar 

  32. Southern, E. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503–517.

    Article  CAS  Google Scholar 

  33. Church, G. and Gilbert, W. 1984. Genomic sequencing. Proc. Natl. Acad. Sci. USA 81: 1991–1995.

    Article  CAS  Google Scholar 

  34. Bagdasarian, M., Lurz, R., Ruckert, B., Franklin, F., Frey, J., Bagdasarian, M. and Timmis, K. 1981. Specific-purpose plasmid cloning vectors II. Broad host range, high copy number, RSF 1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas. Gene 16: 237–247.

    Article  CAS  Google Scholar 

  35. Bolivar, F., Rodriquez, R., Green, P., Betlach, M., Heyneker, H., Boyer, H., Crosa, J. and Falkow, S. 1977. Construction and characterization of new cloning vehicles II. A multipurpose cloning system. Gene 2: 95–113.

    Article  CAS  Google Scholar 

  36. Walbot, V., Dure, L. 1976. Developmental biochemistry of cotton seed embryogenesis and germination VII. Characterization of the cotton genome. J. Mol. Biol. 101: 503–536.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Agracetus, 8520 University Green, Middleton, Wisconsin, 53562

    Paul Umbeck, Gail Johnson, Ken Barton & Will Swain

Authors
  1. Paul Umbeck
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gail Johnson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ken Barton
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Will Swain
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Umbeck, P., Johnson, G., Barton, K. et al. Genetically Transformed Cotton (Gossypium Hirsutum L.) Plants. Nat Biotechnol 5, 263–266 (1987). https://doi.org/10.1038/nbt0387-263

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt0387-263

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing