Abstract
A disarmed derivative of the Ti:R772 cointegrate plasmid pAL969 was used to produce transgenic plants of a commercially important inbred line of oil–producing sunflower. A chimeric gene (neo) composed of the coding sequence of neomycin phosphotransferase (NPT) and 5′ and 3′ sequences from octopine synthase permitted the selection of kanamycin–resistant (Kanr) callus capable of plant regeneration. Kanamycin inhibited plant regeneration, precluding simultaneous plant regeneration and selection for resistance to the antibiotic. Progeny analysis of transformed seedlings indicated Mendelian inheritance of neo. Expression of neo in transformed seedlings and plants indicated that the use of a Ti–derived promoter does not guarantee constitutive expression of the foreign gene. These results demonstrate that the introduction of foreign genes via Agrobacterium–mediated transformation can now be used for agronomic improvement of sunflower.
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Everett, N., Robinson, K. & Mascarenhas, D. Genetic Engineering of Sunflower (Helianthus Annuus L.). Nat Biotechnol 5, 1201–1204 (1987). https://doi.org/10.1038/nbt1187-1201
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DOI: https://doi.org/10.1038/nbt1187-1201
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