Nature Biotechnology

Volume 34 Issue 2, February 2016

Artist's impression of a patch clamp pipette probing the interior of a single neuron in brain tissue. In this issue Cadwell et al. (p 199) and Fuzik et al. (p 175) present methods for the combined electrophysiological, transcriptomic and morphologic profiling of single cells in situ. Credit: Sputnik Animation, Ed Boyden and the McGovern Institute for Brain Research at MIT

Volume 34 Issue 2

Editorial

Research not fit to print

Some biotech companies now eschew traditional publication in peer-reviewed journals. Does it matter?

Editorial 05 Feb 2016

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News

FDA approves 'farmaceutical' drug from transgenic chickens

Cormac Sheridan
News 05 Feb 2016
Obama's cancer moonshot

News 05 Feb 2016
Biotechs target stagnant baldness market

Charles Schmidt
News 05 Feb 2016
CRISPR patents to go on trial

News 05 Feb 2016
Bayer joins CRISPR

News 05 Feb 2016
Proteasome play C4 debuts

News 05 Feb 2016
Direct Genomics revives Helicos sequencing system for China's hospitals

David Cyranoski
News 05 Feb 2016
New partner for Galapagos JAK drug

News 05 Feb 2016
Chimera research under the spotlight

Laura DeFrancesco
News 05 Feb 2016

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Correction

Corrections

Correction 05 Feb 2016

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News

Around the world in a month

News 05 Feb 2016
FDA approves three different multiple myeloma drugs in one month

Mark Ratner
News 05 Feb 2016

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Data Page

2015—another banner year for biotech

Walter Yang
Data Page 05 Feb 2016
Drug pipeline Q4 2015

Laura DeFrancesco
Data Page 05 Feb 2016

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News Feature

Fresh from the biotech pipeline—2015

Novel drug approvals in the United States surpassed even 2014's unprecedented totals. The broad trends are familiar but, as Chris Morrison reports, 2015 had its share of pioneering achievements.

Chris Morrison
News Feature 05 Feb 2016

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Bioentrepreneur

Preparing for crises

How can you prepare for the day when an unexpected event morphs into a crisis, with all the potential negative repercussions for your company's reputation and financial standing?

Mark Kessel
Robert Masella
Bioentrepreneur 05 Feb 2016

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Correspondence

Cas9-triggered chain ablation of cas9 as a gene drive brake

Bing Wu
Liqun Luo
Xiaojing J Gao
Correspondence 05 Feb 2016
Precision and robustness of 2D-NMR for structure assessment of filgrastim biosimilars

Houman Ghasriani
Derek J Hodgson
David A Keire
Correspondence 05 Feb 2016
Evolving Japanese regulations on companion diagnostics

Sumimasa Nagai
Masaaki Urata
Mayumi Shikano
Correspondence 05 Jan 2016

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Patents

Persistent confusion and controversy surrounding gene patents

The development of patent-specific educational resources and prompt resolution of patentability rules unsettled by recent US Supreme Court decisions are urgently needed in the genomics industry.

Christi J Guerrini
Mary A Majumder
Amy L McGuire
Patents 05 Feb 2016
Recent patents in computational biology

Patents 05 Feb 2016

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News & Views

Can CRISPR-Cas9 gene drives curb malaria?

Gene drives in mosquitoes to reduce the spread of malaria move closer, though technical and regulatory hurdles remain.

Luke Alphey
News & Views 05 Feb 2016
Mapping regulatory elements

Regulatory genomic elements can now be studied in their native context using two CRISPR-based high-throughput approaches.

Yuexin Zhou
Wensheng Wei
News & Views 05 Feb 2016
Personalized nutrition through big data

A systems model of glycemic response identifies individually tailored diets that keep blood sugar in check.

Daniel McDonald
Gustavo Glusman
Nathan D Price
News & Views 05 Feb 2016
Research Highlights

News & Views 05 Feb 2016

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Analysis

Identifying recurrent mutations in cancer reveals widespread lineage diversity and mutational specificity

Detection of recurrently mutated nucleotides identifies novel cancer hotspots in an analysis of >11,000 human tumor samples.

Matthew T Chang
Saurabh Asthana
Barry S Taylor
Analysis 30 Nov 2015

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Brief Communication

Antisense oligonucleotide–directed inhibition of nonsense-mediated mRNA decay

Antisense oligonucleotides target genetic disease via gene-specific inhibition of nonsense-mediated mRNA decay.

Tomoki T Nomakuchi
Frank Rigo
Adrian R Krainer
Brief Communication 14 Dec 2015

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Article

High-throughput mapping of regulatory DNA

Cis-regulatory genomic regions necessary for gene expression are revealed by a high-throughput CRISPR-Cas9–based assay.

Nisha Rajagopal
Sharanya Srinivasan
Richard I Sherwood
Article 25 Jan 2016
Integration of electrophysiological recordings with single-cell RNA-seq data identifies neuronal subtypes

Patch-seq reveals new neuronal subtypes by combining electrophysiological and RNA-seq data on single neurons in situ.

János Fuzik
Amit Zeisel
Tibor Harkany
Article 21 Dec 2015
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

Genome-wide sgRNA libraries based on rules for on-target activity improve results of Cas9-based screens and facilitate a further refinement of on- and off-target prediction algorithms.

John G Doench
Nicolo Fusi
David E Root
Article 18 Jan 2016

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Letter

Functional genetic screens for enhancer elements in the human genome using CRISPR-Cas9

CRISPR-Cas9 is used for both enrichment and dropout screens of functional enhancers in human cells.

Gozde Korkmaz
Rui Lopes
Reuven Agami
Letter 11 Jan 2016
Electrophysiological, transcriptomic and morphologic profiling of single neurons using Patch-seq

Patch-seq reveals new neuronal subtypes by combining electrophysiological and RNA-seq data on single neurons in situ.

Cathryn R Cadwell
Athanasia Palasantza
Andreas S Tolias
Letter 21 Dec 2015
Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain

AAV vectors that efficiently transduce the mouse brain after intravenous injection are generated with a CRE-dependent selection system.

Benjamin E Deverman
Piers L Pravdo
Viviana Gradinaru
Letter 01 Feb 2016

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Erratum

Erratum: Analysis of intronic and exonic reads in RNA-seq data characterizes transcriptional and post-transcriptional regulation

Dimos Gaidatzis
Lukas Burger
Michael B Stadler
Erratum 05 Feb 2016
Erratum: Share and share alike

Samuel K Sia
Matthew P Owens
Erratum 05 Feb 2016

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Corrigendum

Correction: Corrigendum: Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining

Takeshi Maruyama
Stephanie K Dougan
Hidde L Ploegh
Corrigendum 05 Feb 2016

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Careers and Recruitment

Fourth-quarter biotech job picture

Michael Francisco
Careers and Recruitment 05 Feb 2016
People

Careers and Recruitment 05 Feb 2016

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