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Editorial

Evolving fixes for drug pricing p1209

doi:10.1038/nbt.3748

Despite an increasingly strident outcry against drug prices in the United States, manufacturers likely face an evolution in the reimbursement landscape, rather than a revolution.


Trump election sends aftershocks through biotech sector pp1211 - 1212

Chris Morrison

doi:10.1038/nbt1216-1211


UK forms a £1-billion life sciences powerhouse p1212

doi:10.1038/nbt1216-1212


Alnylam terminates revusiran program, stock plunges pp1213 - 1214

Ken Garber

doi:10.1038/nbt1216-1213


Pfizer drops phase 3 lipid-lowering antibody p1214

doi:10.1038/nbt1216-1214


Despite slow progress, bispecifics generate buzz pp1215 - 1217

Cormac Sheridan

doi:10.1038/nbt1216-1215


New anti-IL-23 drugs raise hopes for psoriasis plaque clearance pp1218 - 1219

Elie Dolgin

doi:10.1038/nbt1216-1218


Medtronic automated insulin delivery device gets FDA nod p1220

Eric Smalley

doi:10.1038/nbt1216-1220


Around the world in a month p1221

doi:10.1038/nbt1216-1221


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Bioentrepreneur

Building a business

Closing the deal pp1222 - 1225

Sadhana Chitale, Colm Lawler & Scott Macfarlane

doi:10.1038/nbt.3687

A checklist for negotiating robust licensing agreements.


Podcast

First Rounders Podcast: Anu Acharya p1225

doi:10.1038/nbt.3736


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Opinion and Comment

Correspondence

US National Academies report misses the mark pp1226 - 1228

L Val Giddings & Henry Miller

doi:10.1038/nbt.3746


Getting stem cell patients 'on the grid' pp1228 - 1230

Paul Wicks & Jamie Heywood

doi:10.1038/nbt.3740


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Features

America's drug problem pp1231 - 1241

Brady Huggett

doi:10.1038/nbt.3734

Chasing treatments and gouging patients in a bloated, capitalist healthcare system.


Patents

An update on obtaining and enforcing therapeutic antibody patent claims pp1242 - 1244

Theresa Gresl, Ulrich Storz & Colin Sandercock

doi:10.1038/nbt.3735

Therapeutic antibody patent owners face continuing challenges trying to obtain broad patents and enforce them in the United States and Europe.


Recent patents in antibody engineering p1245

doi:10.1038/nbt.3752


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News and Views

Let there be light—at the right place pp1246 - 1247

Lars Hufnagel & Rainer Pepperkok

doi:10.1038/nbt.3738

A light-sheet microscope that automatically corrects optical aberrations enables quantitative imaging of deep tissue.

See also: Research by Royer et al.


Are human oocytes from stem cells next? pp1247 - 1248

Johan E J Smitz & Robert B Gilchrist

doi:10.1038/nbt.3742

The generation of oocytes in a dish from mouse pluripotent cells may be difficult to replicate with human cells.


Research Highlights p1249

doi:10.1038/nbt.3741


Hacking rules for E. coli p1249

Irene Jarchum

doi:10.1038/nbt.3744


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Research

Perspective

Imagining the future of bioimage analysis pp1250 - 1255

Erik Meijering, Anne E Carpenter, Hanchuan Peng, Fred A Hamprecht & Jean-Christophe Olivo-Marin

doi:10.1038/nbt.3722

Modern biological research increasingly relies on image data as a primary source of information in unraveling the cellular and molecular mechanisms of life. The quantity and complexity of the data generated by state-of-the-art microscopes preclude visual or manual analysis and require advanced computational methods to fully explore the wealth of information. In addition to making bioimage analysis more efficient, objective, and reproducible, the use of computers improves the accuracy and sensitivity of the analyses and helps to reveal subtleties that may be unnoticeable to the human eye. Many methods and software tools have already been developed to this end, but there is still a long way to go before biologists can blindly trust automated measurements. Here, we summarize the current state of the art in bioimage analysis and provide a perspective on likely future developments.


Analysis

Measurement of bacterial replication rates in microbial communities pp1256 - 1263

Christopher T Brown, Matthew R Olm, Brian C Thomas & Jillian F Banfield

doi:10.1038/nbt.3704

Replication rates of bacteria in both human and environmental microbiomes are measured without reference genome sequences.


Brief Communications

Single-cell sequencing of the small-RNA transcriptome pp1264 - 1266

Omid R Faridani, Ilgar Abdullayev, Michael Hagemann-Jensen, John P Schell, Fredrik Lanner & Rickard Sandberg

doi:10.1038/nbt.3701

The small RNA transcriptome is sequenced in single cells, facilitating study of microRNAs and other small RNAs.


Articles

Adaptive light-sheet microscopy for long-term, high-resolution imaging in living organisms pp1267 - 1278

Loïc A Royer, William C Lemon, Raghav K Chhetri, Yinan Wan, Michael Coleman, Eugene W Myers & Philipp J Keller

doi:10.1038/nbt.3708

Adaptive light-sheet microscopy improves imaging of live organisms by correcting for optical aberrations in real time.

See also: News and Views by Hufnagel & Pepperkok


Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR–Cas9 library pp1279 - 1286

Shiyou Zhu, Wei Li, Jingze Liu, Chen-Hao Chen, Qi Liao, Ping Xu, Han Xu, Tengfei Xiao, Zhongzheng Cao, Jingyu Peng, Pengfei Yuan, Myles Brown, Xiaole Shirley Liu & Wensheng Wei

doi:10.1038/nbt.3715

Long non-coding RNAs are identified using a high-throughput paired-guide RNA genomic deletion screen.


Letter

Modeling of RNA-seq fragment sequence bias reduces systematic errors in transcript abundance estimation pp1287 - 1291

Michael I Love, John B Hogenesch & Rafael A Irizarry

doi:10.1038/nbt.3682

Estimates of transcript abundance in RNA-seq data are improved by accounting for sample-specific biases.


Errata

Erratum: Top 20 translational researchers of 2015 p1292

Brady Huggett & Kathryn Paisner

doi:10.1038/nbt1216-1292a


Erratum: A spark at the periphery p1292

Emily Waltz

doi:10.1038/nbt1216-1292b


Erratum: Rekindling cancer vaccines p1292

Malorye Allison Branca

doi:10.1038/nbt1216-1292c


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Careers and Recruitment

Interactive and scalable biology cloud experimentation for scientific inquiry and education pp1293 - 1298

Zahid Hossain, Engin W Bumbacher, Alice M Chung, Honesty Kim, Casey Litton, Ashley D Walter, Sachin N Pradhan, Kemi Jona, Paulo Blikstein & Ingmar H Riedel-Kruse

doi:10.1038/nbt.3747

A real-time interactive, fully automated, low-cost and scalable biology cloud experimentation platform could provide access to scientific experimentation for learners and researchers alike.


People

People p1300

doi:10.1038/nbt.3751


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