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Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems

Nature Biotechnology volume 31, pages 684–686 (2013)Cite this article

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CRISPRs are clustered, regularly interspaced, short palindromic repeats present in many bacteria and archaea genomes. Proteins encoded by CRISPR-associated (Cas) genes serve as guardians of the genome, which target foreign DNA at specific sites by means of small CRISPR RNA (crRNA)-guided DNA recognition and degradation1,2,3,4. Recently, several groups described how CRISPR-Cas systems efficiently create site-specific gene modifications in whole organisms such as Streptococcus pneumoniae, Escherichia coli, Danio rerio (zebrafish) and mice, suggesting its potential application in the production of genetically engineered organisms5,6,7, although germline transmission of the mutations remains to be shown. Here, we report the use of CRISPR-Cas systems to generate multiple gene mutations in rats in a germline-competent manner.

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Figure 1: Targeted disruption in the Tet gene loci in rat by means of CRISPR-Cas systems.

References

  1. Jinek, M. et al. Science 337, 816–821 (2012).

    Article  CAS  Google Scholar 

  2. Cho, S.W., Kim, S., Kim, J.M. & Kim, J.S. Nat. Biotechnol. 31, 230–232 (2013).

    Article  CAS  Google Scholar 

  3. Cong, L. et al. Science 339, 819–823 (2013).

    Article  CAS  Google Scholar 

  4. Mali, P. et al. Science 339, 823–826 (2013).

    Article  CAS  Google Scholar 

  5. Hwang, W.Y. et al. Nat. Biotechnol. 31, 227–229 (2013).

    Article  CAS  Google Scholar 

  6. Jiang, W., Bikard, D., Cox, D., Zhang, F. & Marraffini, L.A. Nat. Biotechnol. 31, 233–239 (2013).

    Article  CAS  Google Scholar 

  7. Wang, H. et al. Cell 153, 910–918 (2013).

    Article  CAS  Google Scholar 

  8. Geurts, A.M. et al. Science 325, 433 (2009).

    Article  CAS  Google Scholar 

  9. Tong, C., Li, P., Wu, N.L., Yan, Y. & Ying, Q.L. Nature 467, 211–213 (2010).

    Article  CAS  Google Scholar 

  10. Tesson, L. et al. Nat. Biotechnol. 29, 695–696 (2011).

    Article  CAS  Google Scholar 

  11. Wu, H. & Zhang, Y. Genes Dev. 25, 2436–2452 (2011).

    Article  CAS  Google Scholar 

  12. Gu, T.P. et al. Nature 477, 606–610 (2011).

    Article  CAS  Google Scholar 

  13. Dawlaty, M.M. et al. Dev. Cell 24, 310–323 (2013).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was supported by grants from the National Basic Research Program of China 2012CBA01300, “Strategic Priority Research Program” of the Chinese Academy of Sciences XDA01020101, the National Science Foundation of China 90919060 and the Ministry of Science and Technology of China 2011CBA01101 (all to Q.Z.).

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  1. Wei Li and Fei Teng: These authors contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China

    Wei Li, Fei Teng, Tianda Li & Qi Zhou

  2. University of Chinese Academy of Sciences, Beijing, China

    Fei Teng

Authors
  1. Wei Li
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  2. Fei Teng
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  3. Tianda Li
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  4. Qi Zhou
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Correspondence to Qi Zhou.

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Supplementary Methods, Supplementary Discussion, Supplementary Figures 1–9, Supplementary Tables 1–3 and Supplementary Sequences (PDF 3420 kb)

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Li, W., Teng, F., Li, T. et al. Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems. Nat Biotechnol 31, 684–686 (2013). https://doi.org/10.1038/nbt.2652

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