Efficient genome editing in zebrafish using a CRISPR-Cas system

Journal name:
Nature Biotechnology
Volume:
31,
Pages:
227–229
Year published:
DOI:
doi:10.1038/nbt.2501
Received
Accepted
Published online

Abstract

In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator–like effector nucleases.

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Author information

  1. These authors contributed equally to this work.

    • Woong Y Hwang &
    • Yanfang Fu

Affiliations

  1. Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA.

    • Woong Y Hwang,
    • Randall T Peterson &
    • J-R Joanna Yeh
  2. Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, USA.

    • Yanfang Fu,
    • Deepak Reyon,
    • Morgan L Maeder,
    • Shengdar Q Tsai,
    • Jeffry D Sander &
    • J Keith Joung
  3. Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA.

    • Yanfang Fu,
    • Deepak Reyon,
    • Shengdar Q Tsai,
    • Jeffry D Sander &
    • J Keith Joung
  4. Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, Massachusetts, USA.

    • Morgan L Maeder &
    • J Keith Joung
  5. Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA.

    • Randall T Peterson &
    • J-R Joanna Yeh
  6. Broad Institute, Cambridge, Massachusetts, USA.

    • Randall T Peterson

Contributions

W.Y.H., Y.F., M.L.M., D.R., S.Q.T., J.D.S., R.T.P., J.-R.J.Y. and J.K.J. designed experiments. W.Y.H., Y.F., M.L.M., D.R., S.Q.T. and J.D.S. performed experiments. W.Y.H., Y.F., M.L.M., D.R., S.Q.T., J.D.S., R.T.P., J.-R.J.Y. and J.K.J. wrote the paper.

Competing financial interests

J.K.J. has a financial interest in Transposagen Biopharmaceuticals.

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Supplementary information

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    Supplementary Discussion, Supplementary Methods, Supplementary Figures 1–5 and Supplementary Tables 1–5

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