Vaccine market boosters - pp499 - 501

Cormac Sheridan

doi:10.1038/nbt0609-499

Recent commercial success belies conventional wisdom that vaccines are a low-margin, moribund sector. But will the trend continue? Cormac Sheridan investigates.

Abstract - | Full Text - Vaccine market boosters | PDF (181 KB) - Vaccine market boosters

Can you hear me in the back? - pp502 - 503

Carin Canale

doi:10.1038/bioe.2009.4

PDF (101 KB) - Can you hear me in the back?

Pharma's role is not to bankroll biotech - p504

Martin Mackay

doi:10.1038/nbt0609-504a

Full Text - Pharma's role is not to bankroll biotech | PDF (159 KB) - Pharma's role is not to bankroll biotech

Conflating MTAs and patents - pp504 - 505

Kevin E Noonan

doi:10.1038/nbt0609-504b

Full Text - Conflating MTAs and patents | PDF (210 KB) - Conflating MTAs and patents

Reply to Conflating MTAs and patents - p505

doi:10.1038/nbt0609-505

Full Text - Reply to Conflating MTAs and patents | PDF (214 KB) - Reply to Conflating MTAs and patents

First GM trial in Belgium since 2002 - p506

René Custers

doi:10.1038/nbt0609-506

Full Text - First GM trial in Belgium since 2002 | PDF (163 KB) - First GM trial in Belgium since 2002

A European perspective on immunogenicity evaluation - pp507 - 508

Christian K Schneider, Marisa Papaluca & Pekka Kurki

doi:10.1038/nbt0609-507

Full Text - A European perspective on immunogenicity evaluation | PDF (140 KB) - A European perspective on immunogenicity evaluation

Reflect: augmented browsing for the life scientist - pp508 - 510

Evangelos Pafilis, Seán I O'Donoghue, Lars J Jensen, Heiko Horn, Michael Kuhn, Nigel P Brown & Reinhard Schneider

doi:10.1038/nbt0609-508

Full Text - Reflect: augmented browsing for the life scientist | PDF (264 KB) - Reflect: augmented browsing for the life scientist | Supplementary information

Extrapolating from sequence—the 2009 H1N1 'swine' influenza virus - pp510 - 513

Venkataramanan Soundararajan, Kannan Tharakaraman, Rahul Raman, S Raguram, Zachary Shriver, V Sasisekharan & Ram Sasisekharan

doi:10.1038/nbt0609-510

Full Text - Extrapolating from sequence—the 2009 H1N1 'swine' influenza virus | PDF (620 KB) - Extrapolating from sequence—the 2009 H1N1 'swine' influenza virus | Supplementary information

Science communication reconsidered - pp514 - 518

Tania Bubela, Matthew C Nisbet, Rick Borchelt, Fern Brunger, Cristine Critchley, Edna Einsiedel, Gail Geller, Anil Gupta, Jürgen Hampel, Robyn Hyde-Lay, Eric W Jandciu, S Ashley Jones, Pam Kolopack, Summer Lane, Tim Lougheed, Brigitte Nerlich, Ubaka Ogbogu, Kathleen O'Riordan, Colin Ouellette, Mike Spear, Stephen Strauss, Thushaanthini Thavaratnam, Lisa Willemse & Timothy Caulfield

doi:10.1038/nbt0609-514

As new media proliferate and the public's trust and engagement in science are influenced by industry involvement in academic research, an interdisciplinary workshop provides some recommendations to enhance science communication.

Abstract - | Full Text - Science communication reconsidered | PDF (333 KB) - Science communication reconsidered

Strangled at birth? Forest biotech and the Convention on Biological Diversity - pp519 - 527

Steven H. Strauss, Huimin Tan, Wout Boerjan & Roger Sedjo

doi:10.1038/nbt0609-519

Against the Cartagena Protocol and widespread scientific support for a case-by-case approach to regulation, the Convention on Biological Diversity has become a platform for imposing broad restrictions on research and development of all types of transgenic trees.

Abstract - | Full Text - Strangled at birth? Forest biotech and the Convention on Biological Diversity | PDF (297 KB) - Strangled at birth? Forest biotech and the Convention on Biological Diversity

A day late and a few million dollars short - pp538 - 541

Dianna Goldenson

doi:10.1038/nbt0609-538

The pitfalls of seeking and obtaining a patent term extension.

Abstract - | Full Text - A day late and a few million dollars short | PDF (128 KB) - A day late and a few million dollars short

Recent patent applications in genomic assays - p542

doi:10.1038/nbt0609-542

Full Text - Recent patent applications in genomic assays | PDF (57 KB) - Recent patent applications in genomic assays

Transfection of small RNAs globally perturbs gene regulation by endogenous microRNAs - pp549 - 555

Aly A Khan, Doron Betel, Martin L Miller, Chris Sander, Christina S Leslie & Debora S Marks

doi:10.1038/nbt.1543

Transfection of siRNAs and miRNAs into cells has been observed to generate unexpected effects in the form of gene upregulation. By statistically analyzing published transfection experiments, Khan et al. explain these effects with a model that the transfected RNAs compete with miRNAs naturally expressed by the cell.

Abstract - | Full Text - Transfection of small RNAs globally perturbs gene regulation by endogenous microRNAs | PDF (543 KB) - Transfection of small RNAs globally perturbs gene regulation by endogenous microRNAs | Supplementary information

Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture - pp557 - 559

Michael T Forrester, J Will Thompson, Matthew W Foster, Leonardo Nogueira, M Arthur Moseley & Jonathan S Stamler

doi:10.1038/nbt.1545

Forrester et al. adapt the biotin switch technique for studying reversible protein modification by nitric oxide, by using resin-assisted capture of S-nitrosothiols. This enables more sensitive detection of larger S-nitrosylated proteins and can be coupled with quantitative mass spectrometry to study the kinetics of denitrosylation.

First Paragraph - | Full Text - Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture | PDF (219 KB) - Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture | Supplementary information

Genome sequence of the recombinant protein production host Pichia pastoris - pp561 - 566

Kristof De Schutter, Yao-Cheng Lin, Petra Tiels, Annelies Van Hecke, Sascha Glinka, Jacqueline Weber-Lehmann, Pierre Rouzé, Yves Van de Peer & Nico Callewaert

doi:10.1038/nbt.1544

Pichia pastoris has been a workhorse of protein production for decades. De Schutter et al. present its genomic sequence, which will allow development of improved strains.

Abstract - | Full Text - Genome sequence of the recombinant protein production host Pichia pastoris | PDF (637 KB) - Genome sequence of the recombinant protein production host Pichia pastoris | Supplementary information

Efficient siRNA delivery into primary cells by a peptide transduction domain–dsRNA binding domain fusion protein - pp567 - 571

Akiko Eguchi, Bryan R Meade, Yung-Chi Chang, Craig T Fredrickson, Karl Willert, Nitin Puri & Steven F Dowdy

doi:10.1038/nbt.1541

The delivery to primary cells is a major challenge in the application of siRNAs in biological research. Using a fusion protein consisting of a double-stranded RNA binding domain and a protein transduction domain, Eguchi et al. are able to transfect siRNAs into a wide variety of cells with very high efficiency without cytotoxicity.

First Paragraph - | Full Text - Efficient siRNA delivery into primary cells by a peptide transduction domain–dsRNA binding domain fusion protein | PDF (438 KB) - Efficient siRNA delivery into primary cells by a peptide transduction domain–dsRNA binding domain fusion protein | Supplementary information

MicroRNA-mediated species-specific attenuation of influenza A virus - pp572 - 576

Jasmine T Perez, Alissa M Pham, Maria H Lorini, Mark A Chua, John Steel & Benjamin R tenOever

doi:10.1038/nbt.1542

Traditional methods for modifying viruses to produce live attenuated vaccines are being updated with molecularly targeted approaches. Perez et al. attenuate influenza A viruses by introducing miRNA target sites into the coding region of the viral genome.

First Paragraph - | Full Text - MicroRNA-mediated species-specific attenuation of influenza A virus | PDF (451 KB) - MicroRNA-mediated species-specific attenuation of influenza A virus | Supplementary information

Career interrupted - p577

Mari Paul

doi:10.1038/nbt0609-577

A sound game plan can help you roll with the punches in uncertain times.

Abstract - | Full Text - Career interrupted | PDF (78 KB) - Career interrupted

People - p578

doi:10.1038/nbt0609-578

Full Text - People | PDF (114 KB) - People