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Research
Nature Biotechnology 27, 557–559 (1 June 2009) | doi:10.1038/nbt.1545
Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture
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Abstract
We have modified the biotin switch assay for protein S-nitrosothiols (SNOs), using resin-assisted capture (SNO-RAC). Compared with existing methodologies, SNO-RAC requires fewer steps, detects high-mass S-nitrosylated proteins more efficiently, and facilitates identification and quantification of S-nitrosylated sites by mass spectrometry.
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