Article abstract


Nature Biotechnology 27, 561 - 566 (2009)
Published online: 24 May 2009 | doi:10.1038/nbt.1544

Genome sequence of the recombinant protein production host Pichia pastoris

Kristof De Schutter1,2,7, Yao-Cheng Lin3,4,7, Petra Tiels1,5,7, Annelies Van Hecke1,5, Sascha Glinka6, Jacqueline Weber-Lehmann6, Pierre Rouzé3,4, Yves Van de Peer3,4 & Nico Callewaert1,5

This article is distributed under the terms of the Creative Commons Attribution-Non-Commercial-Share Alike license (http://creativecommons.org/licenses/by-nc-sa/3.0/), which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation, and derivative works must be licensed under the same or similar license.


The methylotrophic yeast Pichia pastoris is widely used for the production of proteins and as a model organism for studying peroxisomal biogenesis and methanol assimilation. P. pastoris strains capable of human-type N-glycosylation are now available, which increases the utility of this organism for biopharmaceutical production. Despite its biotechnological importance, relatively few genetic tools or engineered strains have been generated for P. pastoris. To facilitate progress in these areas, we present the 9.43 Mbp genomic sequence of the GS115 strain of P. pastoris. We also provide manually curated annotation for its 5,313 protein-coding genes.

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  1. Unit for Molecular Glycobiology, Department for Molecular Biomedical Research, VIB, Ghent-Zwijnaarde, Belgium.
  2. Department for Biomedical Molecular Biology, Ghent University, Ghent-Zwijnaarde, Belgium.
  3. Department of Plant Systems Biology, VIB, Ghent-Zwijnaarde, Belgium.
  4. Department of Plant Biotechnology and Genetics, Ghent University, Ghent, Belgium.
  5. Unit for Molecular Glycobiology, L-ProBE, Department of Biochemistry and Microbiology, Ghent University, Ghent-Zwijnaarde, Belgium.
  6. Eurofins MWG Operon, Ebersberg, Germany.
  7. These authors contributed equally to this work.

Correspondence to: Nico Callewaert1,5 e-mail: Nico.Callewaert@dmbr.vib-UGent.be