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Nature Biotechnology 27, 1038–1042 (1 November 2009) | doi:10.1038/nbt.1579

Transcriptional analysis of intracytoplasmically stained, FACS-purified cells by high-throughput, quantitative nuclease protection

Susanne Pechhold , Melissa Stouffer , Gregory Walker , Ralph Martel , Bruce Seligmann , Yan Hang , Roland Stein , David M Harlan & Klaus Pechhold

Analyzing specialized cells in heterogeneous tissues is crucial for understanding organ function in health and disease. Thus far, however, there has been no convenient method for studying gene expression in cells purified by fluorescence-activated cell sorting (FACS) using intracellular markers.