Mark Roth - p13

Charlie Schmidt

doi:10.1038/nbt0109-13

Mark Roth's pioneering work on hydrogen sulfide has spawned Ikaria, a company exploring the molecule's potential to modulate the body's metabolism and perhaps one day turn hibernation into profitable clinical applications.

Abstract - | Full Text - Mark Roth | PDF (112 KB) - Mark Roth

Compensation continues rise - p14

Stacy Lawrence

doi:10.1038/nbt0109-14

Full Text - Compensation continues rise | PDF (265 KB) - Compensation continues rise

Biotech's green gold? - pp15 - 18

Emily Waltz

doi:10.1038/nbt0109-15

Algae have long been touted as a rich and ubiquitous source of renewable fuel but thus far have failed to be economically competitive with other sources of energy. Could new advances change that? Emily Waltz investigates.

Abstract - | Full Text - Biotech's green gold? | PDF (227 KB) - Biotech's green gold?

Four steps to finding the right people - pp19 - 20

Ian Brown

doi:10.1038/bioe.2008.11

PDF (92 KB) - Four steps to finding the right people

Industry and academia must avoid mismatching disclosures - p21

Joseph P Hammang

doi:10.1038/nbt0109-21a

Full Text - Industry and academia must avoid mismatching disclosures | PDF (197 KB) - Industry and academia must avoid mismatching disclosures

Stem cell transplants: the power of peer-to-peer - pp21 - 22

Graham Creasy & Christopher Thomas Scott

doi:10.1038/nbt0109-21b

Full Text - Stem cell transplants: the power of peer-to-peer | PDF (246 KB) - Stem cell transplants: the power of peer-to-peer

Is FAO selling biotech short on biofuels? - pp22 - 23

Jonathan Gressel

doi:10.1038/nbt0109-22

Full Text - Is FAO selling biotech short on biofuels? | PDF (134 KB) - Is FAO selling biotech short on biofuels?

Reply to Is FAO selling biotech short on biofuels? - p23

Terri Raney

doi:10.1038/nbt0109-23

Full Text - Reply to Is FAO selling biotech short on biofuels? | PDF (87 KB) - Reply to Is FAO selling biotech short on biofuels?

PRIME importance of pathology expertise - pp24 - 25

Paul N Schofield, Steve D M Brown, John P Sundberg, Mark Arends, Madhuri V Warren, Pierre Dubus, Michele Ellender, Laurence Fiette, Bjorn Rozell, Leticia Quintanilla-Martinez, Marcello Raspa, Ji-Ying Song, Martin van der Valk & Colin McKerlie

doi:10.1038/nbt0109-24

Full Text - PRIME importance of pathology expertise | PDF (198 KB) - PRIME importance of pathology expertise

Quality by design for biopharmaceuticals - pp26 - 34

Anurag S Rathore & Helen Winkle

doi:10.1038/nbt0109-26

The US Food and Drug Administration's 'quality by design' approach is likely to transform the manufacture of biologics.

Abstract - | Full Text - Quality by design for biopharmaceuticals | PDF (557 KB) - Quality by design for biopharmaceuticals

Patents versus patenting: implications of intellectual property protection for biological research - pp36 - 40

Zhen Lei, Rakhi Juneja & Brian D Wright

doi:10.1038/nbt0109-36

A new survey shows scientists consider the proliferation of intellectual property protection to have a strongly negative effect on research.

Abstract - | Full Text - Patents versus patenting: implications of intellectual property protection for biological research | PDF (158 KB) - Patents versus patenting: implications of intellectual property protection for biological research | Supplementary information

Recent patent applications in antibody fragments - p41

doi:10.1038/nbt0109-41

Full Text - Recent patent applications in antibody fragments | PDF (58 KB) - Recent patent applications in antibody fragments

Understanding the physical properties that control protein crystallization by analysis of large-scale experimental data - pp51 - 57

W Nicholson Price II, Yang Chen, Samuel K Handelman, Helen Neely, Philip Manor, Richard Karlin, Rajesh Nair, Jinfeng Liu, Michael Baran, John Everett, Saichiu N Tong, Farhad Forouhar, Swarup S Swaminathan, Thomas Acton, Rong Xiao, Joseph R Luft, Angela Lauricella, George T DeTitta, Burkhard Rost, Gaetano T Montelione & John F Hunt

doi:10.1038/nbt.1514

The physical properties that determine the propensity of a protein to form a well-ordered crystal suitable for structure determination are poorly understood. An analysis of large-scale crystallization results generated by a structural genomics consortium highlights the importance of low-entropy surface features capable of mediating protein-protein interactions.

Abstract - | Full Text - Understanding the physical properties that control protein crystallization by analysis of large-scale experimental data | PDF (329 KB) - Understanding the physical properties that control protein crystallization by analysis of large-scale experimental data | Supplementary information

Intravascular AAV9 preferentially targets neonatal neurons and adult astrocytes - pp59 - 65

Kevin D Foust, Emily Nurre, Chrystal L Montgomery, Anna Hernandez, Curtis M Chan & Brian K Kaspar

doi:10.1038/nbt.1515

Foust et al. describe a viral vector that crosses the blood-brain barrier, providing a non-invasive method for delivering therapeutic genes to the central nervous system. A single intravascular injection of AAV9 results in widespread transduction of astrocytes in adult mice and of astrocytes and neurons in neonatal mice.

Abstract - | Full Text - Intravascular AAV9 preferentially targets neonatal neurons and adult astrocytes | PDF (982 KB) - Intravascular AAV9 preferentially targets neonatal neurons and adult astrocytes | Supplementary information

See also: News and Views by Lowenstein

PeakSeq enables systematic scoring of ChIP-seq experiments relative to controls - pp66 - 75

Joel Rozowsky, Ghia Euskirchen, Raymond K Auerbach, Zhengdong D Zhang, Theodore Gibson, Robert Bjornson, Nicholas Carriero, Michael Snyder & Mark B Gerstein

doi:10.1038/nbt.1518

Repetitive sequences and chromatin accessibility can confound scoring of chromatin immunoprecipitation data generated by high–throughput sequencing. Using data sets they produce for human RNA polymerase II and the transcription factor STAT1, Rozowsky et al. compensate for these biases by correcting for 'mappability' and normalizing the data against an input–DNA control.

Abstract - | Full Text - PeakSeq enables systematic scoring of ChIP-seq experiments relative to controls | PDF (859 KB) - PeakSeq enables systematic scoring of ChIP-seq experiments relative to controls | Supplementary information

Bead-based profiling of tyrosine kinase phosphorylation identifies SRC as a potential target for glioblastoma therapy - pp77 - 83

Jinyan Du, Paula Bernasconi, Karl R Clauser, D R Mani, Stephen P Finn, Rameen Beroukhim, Melissa Burns, Bina Julian, Xiao P Peng, Haley Hieronymus, Rebecca L Maglathlin, Timothy A Lewis, Linda M Liau, Phioanh Nghiemphu, Ingo K Mellinghoff, David N Louis, Massimo Loda, Steven A Carr, Andrew L Kung & Todd R Golub

doi:10.1038/nbt.1513

Du et al. describe a bead-based method for high-throughput detection of phosphorylated tyrosine kinases and use it to profile 130 human cancer lines. They show that the tyrosine kinase SRC is frequently activated in glioblastoma cells and that a SRC inhibitor blocks the growth of glioblastoma tumors.

First Paragraph - | Full Text - Bead-based profiling of tyrosine kinase phosphorylation identifies SRC as a potential target for glioblastoma therapy | PDF (670 KB) - Bead-based profiling of tyrosine kinase phosphorylation identifies SRC as a potential target for glioblastoma therapy | Supplementary information

Analysis of histone 2B-GFP retention reveals slowly cycling hematopoietic stem cells - pp84 - 90

Adlen Foudi, Konrad Hochedlinger, Denille Van Buren, Jeffrey W Schindler, Rudolf Jaenisch, Vincent Carey & Hanno Hock

doi:10.1038/nbt.1517

Foudi et al. report a method for monitoring the turnover of hematopoietic stem cells that has several advantages over BrdU labeling. Using drug-inducible expression of a histone 2B–GFP fusion protein, which permits a more sensitive analysis of division history, the authors detect hematopoietic stem cells that cycle at a very slow rate.

First Paragraph - | Full Text - Analysis of histone 2B-GFP retention reveals slowly cycling hematopoietic stem cells | PDF (1,296 KB) - Analysis of histone 2B-GFP retention reveals slowly cycling hematopoietic stem cells | Supplementary information

Characterization of human embryonic stem cells with features of neoplastic progression - pp91 - 97

Tamra E Werbowetski-Ogilvie, Marc Bossé, Morag Stewart, Angelique Schnerch, Veronica Ramos-Mejia, Anne Rouleau, Tracy Wynder, Mary-Jo Smith, Steve Dingwall, Tim Carter, Christopher Williams, Charles Harris, Joanna Dolling, Christopher Wynder, Doug Boreham & Mickie Bhatia

doi:10.1038/nbt.1516

Werbowetski-Ogilvie et al. show that cultured human embryonic stem (hES) cells that appear normal may in fact be partially transformed. Two such lines are found to have aberrant growth and differentiation properties and submicroscopic chromosomal abnormalities.

First Paragraph - | Full Text - Characterization of human embryonic stem cells with features of neoplastic progression | PDF (1,162 KB) - Characterization of human embryonic stem cells with features of neoplastic progression | Supplementary information

Executive compensation at private life science companies in troubled economic times - pp98 - 99

Bruce Rychlik & Mike DiPierro

doi:10.1038/nbt0109-98

The upward compensation trends reflected in our survey data, collected in the spring of 2008, stand in stark contrast to the economic conditions since October.

Abstract - | Full Text - Executive compensation at private life science companies in troubled economic times | PDF (208 KB) - Executive compensation at private life science companies in troubled economic times

People - p100

doi:10.1038/nbt0109-100

Full Text - People | PDF (112 KB) - People