Research abstract
Article abstract
Nature Biotechnology 26, 685 - 694 (2008)
Published online: 25 May 2008 | doi:10.1038/nbt1408
Proteome-derived, database-searchable peptide libraries for identifying protease cleavage sites
Oliver Schilling1,2 & Christopher M Overall1
Abstract
We introduce human proteome–derived, database-searchable peptide libraries for characterizing sequence-specific protein interactions. To identify endoprotease cleavage sites, we used peptides in such libraries with protected primary amines to simultaneously determine sequence preferences on the N-terminal (nonprime P) and C-terminal (prime P') sides of the scissile bond. Prime-side cleavage products were tagged with biotin, isolated and identified by tandem mass spectrometry, and the corresponding nonprime-side sequences were derived from human proteome databases using bioinformatics. Identification of hundreds to over 1,000 individual cleaved peptides allows the consensus protease cleavage site and subsite cooperativity to be readily determined from P6 to P6'. For the highly specific GluC protease, >95% of the 558 cleavage sites identified displayed the canonical selectivity. For the broad-specificity matrix metalloproteinase 2, >1,200 peptidic cleavage sites were identified. Profiling of HIV protease 1, caspase 3, caspase 7, cathepsins K and G, elastase and thrombin showed that this approach is broadly applicable to all mechanistic classes of endoproteases.
- The UBC Centre for Blood Research, Departments of Oral Biological and Medical Sciences, and Biochemistry and Molecular Biology, 4.401 Life Sciences Institute, 2350 Health Sciences Mall, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
- Present address: Institute of Molecular Medicine and Cell Research, University of Freiburg, Stefan-Meier-Strasse 17, D-79104 Freiburg, Germany.
Correspondence to: Christopher M Overall1 e-mail: chris.overall@ubc.ca
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