Research abstract
Article abstract
Nature Biotechnology 26, 317 - 325 (2008)
Published online: 17 February 2008 | Corrected online: 16 May 2008 | doi:10.1038/nbt1385
There is a Corrigenda (June 2008) associated with this Article.
Direct multiplexed measurement of gene expression with color-coded probe pairs
Gary K Geiss1, Roger E Bumgarner2, Brian Birditt1, Timothy Dahl1, Naeem Dowidar1, Dwayne L Dunaway1, H Perry Fell1, Sean Ferree1, Renee D George1,5, Tammy Grogan1, Jeffrey J James1, Malini Maysuria1, Jeffrey D Mitton1, Paola Oliveri3,5, Jennifer L Osborn1,5, Tao Peng2, Amber L Ratcliffe1, Philippa J Webster1, Eric H Davidson3, Leroy Hood5 & Krassen Dimitrov4,5
Abstract
We describe a technology, the NanoString nCounter gene expression system, which captures and counts individual mRNA transcripts. Advantages over existing platforms include direct measurement of mRNA expression levels without enzymatic reactions or bias, sensitivity coupled with high multiplex capability, and digital readout. Experiments performed on 509 human genes yielded a replicate correlation coefficient of 0.999, a detection limit between 0.1 fM and 0.5 fM, and a linear dynamic range of over 500-fold. Comparison of the NanoString nCounter gene expression system with microarrays and TaqMan PCR demonstrated that the nCounter system is more sensitive than microarrays and similar in sensitivity to real-time PCR. Finally, a comparison of transcript levels for 21 genes across seven samples measured by the nCounter system and SYBR Green real-time PCR demonstrated similar patterns of gene expression at all transcript levels.
- NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA.
- The Department of Microbiology, Box 358070, University of Washington, Seattle, Washington 98195, USA.
- Division of Biology 156-29, California Institute of Technology, Pasadena, California 91125, USA.
- The Institute of Systems Biology, 1441 N. 34th St., Seattle, Washington 98103, USA.
- Present addresses: Department of Genome Sciences, Box 355065, University of Washington, Seattle, Washington 98195, USA (R.D.G.), Department of Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK (P.O.), Department of Bioengineering, Box 355061, University of Washington, Seattle, Washington 98195, USA (J.L.O.) and Australian Institute for Bioengineering and Nanotechnology, Building 75—Cnr of College and Cooper Road, The University of Queensland, Brisbane QLD 4072 Australia (K.D.)
Correspondence to: Gary K Geiss1 e-mail: ggeiss@nanostring.com
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