Research abstract

Letter abstract


Nature Biotechnology 26, 1373 - 1378 (2008)
Published online: 16 November 2008 | doi:10.1038/nbt.1507

Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of blood

Rong Fan1,2,3,5, Ophir Vermesh1,2,3,5, Alok Srivastava1,4, Brian K H Yen1,2,3, Lidong Qin1,2,3, Habib Ahmad1,2,3, Gabriel A Kwong1,2,3, Chao-Chao Liu1,2,3, Juliane Gould1,2,3, Leroy Hood1,4 & James R Heath1,2,3

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As the tissue that contains the largest representation of the human proteome1, blood is the most important fluid for clinical diagnostics2, 3, 4. However, although changes of plasma protein profiles reflect physiological or pathological conditions associated with many human diseases, only a handful of plasma proteins are routinely used in clinical tests. Reasons for this include the intrinsic complexity of the plasma proteome1, the heterogeneity of human diseases and the rapid degradation of proteins in sampled blood5. We report an integrated microfluidic system, the integrated blood barcode chip that can sensitively sample a large panel of protein biomarkers over broad concentration ranges and within 10 min of sample collection. It enables on-chip blood separation and rapid measurement of a panel of plasma proteins from quantities of whole blood as small as those obtained by a finger prick. Our device holds potential for inexpensive, noninvasive and informative clinical diagnoses, particularly in point-of-care settings.

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  1. NanoSystems Biology Cancer Center, California Institute of Technology, MC 127-72, 1200 E. California Blvd., Pasadena, California 91125, USA
  2. Kavli Nanoscience Institute, California Institute of Technology, MC 127-72, 1200 E. California Blvd., Pasadena, California 91125, USA
  3. Division of Chemistry and Chemical Engineering, California Institute of Technology, MC 127-72, 1200 E. California Blvd., Pasadena, California 91125, USA.
  4. Instititue for Systems Biology, 1441 North 34th St., Seattle, Washington 98103-8904, USA.
  5. These authors contributed equally to this work.

Correspondence to: James R Heath1,2,3 e-mail: heath@caltech.edu



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