Brief Communications abstract
Nature Biotechnology 25, 217 - 219 (2007)
Published online: 28 January 2007 | doi:10.1038/nbt1277
Artificial reporter gene providing MRI contrast based on proton exchange
Assaf A Gilad1,2, Michael T McMahon1,3, Piotr Walczak1,2, Paul T Winnard, Jr1, Venu Raman1,4, Hanneke W M van Laarhoven1,2,5, Cynthia M Skoglund1,2, Jeff W M Bulte1,2 & Peter C M van Zijl1,3
Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water relaxation to gain contrast. We designed a nonmetallic, biodegradable, lysine rich–protein (LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. This endogenous contrast, based on transfer of radiofrequency labeling from the reporter's amide protons to water protons, can be switched on and off.
- The Russell H. Morgan Department of Radiology and Radiological Science, Neurosection, Division of MR Research, Traylor Building Rm. 217, 720 Rutland Ave., Baltimore, Maryland 21205, USA.
- Institute for Cell Engineering, Kennedy Krieger Institute, Baltimore, Maryland 21205, USA.
- Institute for Oncology, The Johns Hopkins University School of Medicine, Kennedy Krieger Institute, Baltimore, Maryland 21205, USA.
- F.M. Kirby Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, Maryland 21205, USA.
- Department of Medical Oncology 452, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands.
Correspondence to: Peter C M van Zijl1,3 e-mail: pvanzijl@mri.jhu.edu
Correspondence to: Jeff W M Bulte1,2 e-mail: jwmbulte@mri.jhu.edu
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