Research abstract

Article abstract


Nature Biotechnology 25, 100 - 106 (2007)
Published online: 7 January 2007 | doi:10.1038/nbt1274

Isolation of amniotic stem cell lines with potential for therapy

Paolo De Coppi1,3, Georg Bartsch Jr1,3, M Minhaj Siddiqui1, Tao Xu1, Cesar C Santos1, Laura Perin1, Gustavo Mostoslavsky2, Angéline C Serre2, Evan Y Snyder2, James J Yoo1, Mark E Furth1, Shay Soker1 & Anthony Atala1


Stem cells capable of differentiating to multiple lineages may be valuable for therapy. We report the isolation of human and rodent amniotic fluid–derived stem (AFS) cells that express embryonic and adult stem cell markers. Undifferentiated AFS cells expand extensively without feeders, double in 36 h and are not tumorigenic. Lines maintained for over 250 population doublings retained long telomeres and a normal karyotype. AFS cells are broadly multipotent. Clonal human lines verified by retroviral marking were induced to differentiate into cell types representing each embryonic germ layer, including cells of adipogenic, osteogenic, myogenic, endothelial, neuronal and hepatic lineages. Examples of differentiated cells derived from human AFS cells and displaying specialized functions include neuronal lineage cells secreting the neurotransmitter L-glutamate or expressing G-protein-gated inwardly rectifying potassium channels, hepatic lineage cells producing urea, and osteogenic lineage cells forming tissue-engineered bone.

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  1. Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC, 27157-1094, USA.
  2. Children's Hospital and Harvard Medical School, 300 Longwood Avenue, Boston, Massachusetts, 02115, USA.
  3. These authors contributed equally to this work.

Correspondence to: Anthony Atala1 e-mail: aatala@wfubmc.edu



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