Nature Biotechnology 24, 351 - 357 (2006)
Published online: 26 February 2006; | doi:10.1038/nbt1197
CD30 is a survival factor and a biomarker for transformed human pluripotent stem cellsDaniella Herszfeld1, 5, Ernst Wolvetang1, 5, Emma Langton-Bunker1, Tung-Liang Chung1, Adam A Filipczyk1, Souheir Houssami1, Pegah Jamshidi1, Karen Koh1, Andrew L Laslett1, Anna Michalska1, Linh Nguyen1, Benjamin E Reubinoff1, 4, Irene Tellis1, Jonathan M Auerbach2, Carol J Ording2, Leendert H J Looijenga3
& Martin F Pera11
Monash Institute of Medical Research, Monash University, and the Australian Stem Cell Centre, Bldg. 75 STRIP, Wellington Road, Clayton, Victoria 3800 Australia. 2
Stem Cell Center, American Type Culture Collection, 10801 University Boulevard, Manassas, Virginia 20110, USA. 3
Pathology/Lab. Exp. Patho-Oncology, Erasmus MC–University Medical Center Rotterdam, Daniel den Hoed Cancer Center, Josephine Nefkens Institute, Building Be, Room 430b, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands. 4
Present address: The Hadassah Human Embryonic Stem Cell Research Center, The Goldyne Savad Institute of Gene Therapy and The Department of Gynecology, Hadassah University Hospital, Ein-Kerem, P.O.B. 12000, Jerusalem 91120, Israel. 5
These authors contributed equally to this work.
Correspondence should be addressed to Martin F Pera martin.pera@med.monash.edu.au M83554M83554M83554D86042D86042The application of human embryonic stem (hES) cells in regenerative medicine will require rigorous quality control measures to ensure the safety of hES cell–derived grafts. During propagation in vitro, hES cells can acquire cytogenetic abnormalities1,
2,
3 as well as submicroscopic genetic lesions, such as small amplifications or deletions4. Many of the genetic abnormalities that arise in hES cell cultures are also implicated in human cancer development. The causes of genetic instability of hES cells in culture are poorly understood, and commonly used cytogenetic methods for detection of abnormal cells are capable only of low-throughput analysis on small numbers of cells. The identification of biomarkers of genetic instability in hES cells would greatly facilitate the development of culture methods that preserve genomic integrity. Here we show that CD30, a member of the tumor necrosis factor receptor superfamily, is expressed on transformed but not normal hES cells, and that CD30 expression protects hES cells against apoptosis.
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