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Research
Nature Biotechnology 24, 185–187 (1 March 2006) | doi:10.1038/nbt1177
Derivation of human embryonic stem cells in defined conditions
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Abstract
We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material.
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