Nature Biotechnology
- 24, 1253 - 1254 (2006)
Published online: 1 October 2006; | doi:10.1038/nbt1249
Protein chip fabrication by capture of nascent polypeptidesSheng-Ce Tao1, 2 & Heng Zhu1, 21
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA. 2
The HiT Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Correspondence should be addressed to Heng Zhu hzhu4@jhmi.edu The most challenging step in protein microarray fabrication is high-throughput production of proteins. Here we report two similar strategies to fabricate protein chips through capture onto a solid surface of the nascent polypeptides during translation of synthetic or in vitro–transcribed RNAs. Using these approaches, we efficiently fabricated both peptide and protein microarrays at relatively high density. We further demonstrated that such protein chips can be used to analyze protein activity.
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