Phage display of antibody libraries is an undeniably powerful technique for isolating highly specific antibodies. However, in the most widely used systems only a small percentage of the phage population carries an antibody, so the libraries are large and unwieldy to screen. On page 75, Rondot et al. describe a new helper phage system that boosts the number of single-chain antibody fragments (scFv) presented on the filamentous bacteriophage by more than two orders of magnitude and show that it significantly increases the number of antigen hits from a given library.
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Sinclair, M. Technical Reports. Nat Biotechnol 19, 8 (2001). https://doi.org/10.1038/83622
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DOI: https://doi.org/10.1038/83622