Nature Biotechnology
16, 1365 - 1369 (1998)
doi:10.1038/4350
The tomato Mi-1 gene confers resistance to both root-knot nematodes
and potato aphidsPieter Vos†1, Guus Simons†1, Taco Jesse1, Jelle Wijbrandi1, Leo Heinen1, René Hogers1, Adri Frijters1, John Groenendijk1, Paul Diergaarde1, Martin Reijans1, Joke Fierens-Onstenk1, Michiel de Both1, Johan Peleman1, Tsvetana Liharska‡2, Jan Hontelez2
& Marc Zabeau§11
Keygene N.V., Agro Business Park, P.O.
Box 216, 6700 AE Wageningen, The Netherlands.
†, These authors contributed equally to this work. §
Present address: Helix, Onafhankelijkheidslaan 38, 9000 Gent, Belgium.
2
Department of Molecular Biology, Wageningen Agricultural
University, Dreyenlaan 3, 6709 DB Wageningen,
The Netherlands. ‡Present address: Department
of Genetics, Free University of Amsterdam, De Boelelaan 1087, 1081 HV Amsterdam,
The Netherlands.
Correspondence should be addressed to Pieter Vos† keygene@euronet.nlagricultural engineeringplant biology
Mi-1, a Lycopersicon peruvianum gene conferring resistance
to the agricultural pests, root-knot nematodes, and introgressed into tomato,
has been cloned using a selective restriction fragment amplification based
strategy. Complementation analysis of a susceptible tomato line with a 100
kb cosmid array yielded a single cosmid clone capable of conferring resistance
both to the root-knot nematode Meloidogyne incognita and to an unrelated
pathogen, the potato aphid Macrosiphum euphorbiae. This resistance
was stable. The Mi-1 gene encodes a protein sharing structural features
with the nucleotide-binding site leucine-rich repeat−containing type
of plant resistance genes.
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