Bio/Technology
10, 1020 - 1025 (1992)
doi:10.1038/nbt0992-1020
Heterologous Encapsidation of Potato Virus Y Strain O (PVYO) With The Transgenic Coat Protein Of PVY Strain N (PVYN) In Solanum Tuberosum Cv. BintjeLaurent Farinelli1, 2, *, Pia Malnoë1, 2, *
& Gérald F. Collet1
1Station Fédérate de Recherches Agronomiques de Changins, CH-1260 Nyon, Switzerland.
2Université de Genève, Département de Biologie Végétale, 30 quai Ernest-Ansermet, CH-1205 Genève, Switzerland.
*Corresponding authors. Potato plants transgenic for the coat protein (CP) gene of potato virus Y strain N (PVYN) were examined for the accumulation of the transgenic PVYN CP. The protein remained undetectable in healthy plants of lines Bt6 and Bt10 that are completely or partially resistant to this virus, but accumulated when the plants were infected by a related strain, PVYO. One possible explanation was that the transgenic CP is stabilized by integration into the capsid of the infecting virus. To investigate this, virus particles originating from these plants were examined by immuno−electron microscopy. Heterologous encapsidation was observed between the infecting PVYO virus and the transgenic PVYN CP. These results have implications for the understanding of the mechanism of CP−mediated protection and assessing its impact on agricultural ecosystems.
REFERENCES
- Weideman, H.L. 1988. Importance and control of potato virus YN (PVYN) in seed potato production. Potato Res. 31: 85−94.
- Hollings, M. and Brunt, A.A. 1981. Potyviruses, p. 731−807. In: Handbook of Plant Virus Infections and Comparative Diagnosis. E. Kurstak (Ed.). Elsevier/North-Holland Biomedical Press, Amsterdam.
- Riechmann, J.L., Laín, S. and Garcfa, J.A. 1992. Highlights and prospects of potyvirus molecular biology. J. Gen. Virol. 73: 1−16. | PubMed | ISI | ChemPort |
- Beachy, R.N., Loesch-Fries, S. and Turner, N.E. 1990. Coat protein-mediated resistance against virus infection. Ann. Rev. Phytopathol. 28: 451−474. | Article | ISI | ChemPort |
- Nejidat, A., Clark, W.G. and Beachy, R.N. 1990. Engineered resistance against plant virus diseases. Physiol. Plant. 80: 662−668. | Article | ISI |
- van den Elzen, P.J.M., Huisman, M.J., Willink, D.P.-L., Jongedijk, E., Hoekema, A. and Cornelissen, B.J.C. 1989. Engineering virus resistance in agricultural crops. Plant Mol. Biol. 13: 337−346. | PubMed | ChemPort |
- Powell, P.A., Sanders, P.R., Turner, N.E., Fraley, R.T. and Beachy, R.N. 1990. Protection against Tobacco Mosaic Virus infection in transgenic plants requires accumulation of coat protein rather than coat protein RNA sequences. Virology 175: 124−130. | Article | PubMed | ISI | ChemPort |
- Lawson, C., Kaniewski, W., Haley, L., Rozman, R., Newell, C., Sanders, P. and Turner, N.E. 1990. Engineering resistance to mixed virus infection in a commercial potato cultivar: resistance to potato virus X and potato virus Y in transgenic Russet Burbank. Bio/Technology 8: 127−134. | PubMed | ISI | ChemPort |
- Golemboski, D.B., Lomonossoff, G. and Zaitlin, M. 1990. Plants transformed with a tobacco mosaic virus nonstructural gene sequence are resistant to the virus. Proc. Natl. Acad. Sci. USA 87: 6311−6315. | PubMed | ChemPort |
- de Zoeten, G.A. 1991. Risk assessment: do we let history repeat itself? Phytopathol. 81: 585−586. | ChemPort |
- Rochow, W.F. 1970. Barley yellow dwarf virus: Phenotypic mixing and vector specificity. Science 167: 875−878. | ISI |
- Bourdin, D. and Lecoq, H. 1991. Evidence that heteroencapsidation between two potyviruses is involved in aphid transmission of a non-aphid-transmissible isolate from mixed infections. Phytopathol. 81: 1459−1464. | ISI |
- Osbourn, J.K., Sakar, S. and Wilson, M.A. 1990. Complementation of coat protein-defective TMV mutants in transgenic tobacco plants expressing TMV coat protein. Virology 179: 921−925. | Article | PubMed | ISI | ChemPort |
- Holt, C.A. and Beachy, R.N. 1991. In vivo complementation of infectious transcripts from mutant Tobacco Mosaic Virus cDNAs in transgenic plants. Virology 181: 109−117. | Article | PubMed | ISI | ChemPort |
- King, A.M.Q., McCahon, D., Slade, W.R. and Newman, J.W.I. 1982. Recombination in RNA. Cell 29: 921−928. | Article | PubMed | ISI | ChemPort |
- Bujarski, J.J. and Kaesberg, P. 1986. Genetic recombination between RNA components of a multipartite plant virus. Nature 321: 528−531. | PubMed | ISI | ChemPort |
- Allison, R., Thompson, C. and Ahlquist, P. 1990. Regeneration of a functional RNA virus genome by recombination between deletion mutants and requirement for cowpea chlorotic mottle virus 3a and coat genes for systemic infection. Proc. Natl. Acad. Sci. USA 87: 1820−1824. | PubMed | ChemPort |
- Angenent, G.C., Posthumus, E., Brederode, F.T. and Bol, J.F. 1989. Genome structure of tobacco rattle virus strain PLBV: evidence on the occurrence of RNA recombination among tobraviruses. Virology 171: 271−274. | Article | PubMed | ISI | ChemPort |
- Rao, A.L.N. and Hall, T.C. 1990. Requirement for a viral trans-acting factor encoded by brome mosaic virus RNA 2 provides strong selection in vivo for functional recombinants. J. Virol. 64: 2437−2441. | PubMed | ISI | ChemPort |
- Zaitlin, M. 1976. Viral cross protection: more understanding is needed. Phytopathol. 66: 382−383. | ISI |
- Gerber, M. and Sarkar, S. 1989. The coat protein of tobacco mosaic virus does not play a significant role for cross-protection. J. Phythopath. 124: 323−331.
- Gera, A., Loebenstein, G. and Raccah, B. 1979. Protein coats of two strains of cucumber mosaic virus affects transmission by Aphis gossypii. Phytopathol. 69: 396−399. | ISI | ChemPort |
- Hull, R. 1977. Particle differences related to aphid-transmissibility of a plant virus. J. Gen. Virol. 34: 183−187. | ISI |
- Pirone, T.P. and Thornbury, D.W. 1983. Role of virion and helper component in regulating aphid transmission of tobacco etch virus. Phytopathol. 73: 872−875. | ISI |
- Pirone, T.P. 1977. Accessory factors in non-persistent virus transmission, p. 221−235. In: Aphids as Virus Vectors. K. Maramorosch and H. Koprowski (Eds.). Academic Press, New York.
- Lindbo, J.A. and Dougherty, W.G. 1992. Pathogen-derived resistance to a potyvirus: Immune and resistant phenotypes in transgenic tobacco expressing altered forms of a potyvirus coat protein nucleotide sequence. Mol. Plant Microbe Interact. 5: 144−153. | PubMed | ISI | ChemPort |
- Gugerli, P. and Fries, P. 1983. Characterization of monoclonal antibodies to Potato Virus Y and their use for virus detection. J. Gen. Virol. 64: 2471−2477. | ISI |
- Koncz, C., Martini, N., Mayerhofer, R., Koncz-Kalman, Z., Körber, H., Redei, G.P. and Schell, J. 1989. High-frequency T-DNA-mediated gene tagging in plants. Proc. Natl. Acad. Sci. USA 86: 8467−8471. | PubMed | ChemPort |
- Gugerli, P. 1986. Potato viruses, p. 430−446. In: Methods of Enzymatic Analysis. Volume XI: Antigens and Antibodies 2. H.U. Bergmeyer (Ed.). VCH Verlagsgesellschaft, Weinheim, FRG.
- Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248−254. | Article | PubMed | ISI | ChemPort |
- Laemmli, U.K. 1970. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227: 680−685. | PubMed | ISI | ChemPort |
- Fling, S.P. and Gregerson, D.S. 1986. Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea. Anal. Biochem. 155: 83−88. | PubMed | ISI | ChemPort |
- Harlow, E. and Lane, D. 1988. Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
- Luciano, C.S., Murphy, J.F., Rhoads, R.E. and Shaw, J.G. 1991. VPg mediated aggregation of potyviral RNA. J. Gen. Virol. 72: 205−208. | PubMed | ISI | ChemPort |
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