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Volume 618 Issue 7967, 29 June 2023

Shining example

RNA molecules can adopt complex 3D structures, but whether DNA can self-assemble into similar 3D folded structures has been less clear. In this week’s issue, Luiz Passalacqua and his colleagues use a DNA mimic of green fluorescent protein (GFP) to investigate this question. Called Lettuce, the mimic can induce fluorescence in a number of fluorophores. The researchers used X-ray crystallography and cryogenic electron microscopy to characterize complexes of Lettuce with several fluorophores. They found that Lettuce folds in an unusual way to connect with a fluorophore and induce fluorescence, suggesting that DNA can self-assemble into complex 3D structures, and shedding light on how the structural organization of DNA helps confer biochemical function. A Lettuce–fluorophore complex is depicted on the cover (centre) along with GFP (top) and an RNA aptamer with a similar function called Spinach (bottom right).

Cover image: Ethan Tyler/NIH Medical Arts Branch

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    • X-ray crystallography and cryo-electron microscopy analyses of Lettuce—a DNA mimic of GFP—bound to various fluorophores reveal previously unknown structures of DNA that rival analogous RNAs in complexity.

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  • Malaria incidence and mortality rates have hardly changed since 2015.

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