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Article
Nature 456, 459-463 (27 November 2008) | doi:10.1038/nature07519; Received 27 August 2008; Accepted 2 October 2008
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Gibberellin-induced DELLA recognition by the gibberellin receptor GID1
Kohji Murase1,2,3, Yoshinori Hirano1,3, Tai-ping Sun2 & Toshio Hakoshima1
- Structural Biology Laboratory, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan
- Department of Biology, Duke University, Durham, North Carolina 27708, USA
- These authors contributed equally to this work.
Correspondence to: Toshio Hakoshima1 Correspondence and requests for materials should be addressed to T.H. (Email: hakosima@bs.naist.jp).
Abstract
Gibberellins control a range of growth and developmental processes in higher plants and have been widely used in the agricultural industry. By binding to a nuclear receptor, GIBBERELLIN INSENSITIVE DWARF1 (GID1), gibberellins regulate gene expression by promoting degradation of the transcriptional regulator DELLA proteins, including GIBBERELLIN INSENSITIVE (GAI). The precise manner in which GID1 discriminates and becomes activated by bioactive gibberellins for specific binding to DELLA proteins remains unclear. Here we present the crystal structure of a ternary complex of Arabidopsis thaliana GID1A, a bioactive gibberellin and the amino-terminal DELLA domain of GAI. In this complex, GID1A occludes gibberellin in a deep binding pocket covered by its N-terminal helical switch region, which in turn interacts with the DELLA domain containing DELLA, VHYNP and LExLE motifs. Our results establish a structural model of a plant hormone receptor that is distinct from the mechanism of the hormone perception and effector recognition of the known auxin receptors.
- Structural Biology Laboratory, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan
- Department of Biology, Duke University, Durham, North Carolina 27708, USA
- These authors contributed equally to this work.
Correspondence to: Toshio Hakoshima1 Correspondence and requests for materials should be addressed to T.H. (Email: hakosima@bs.naist.jp).
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