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Nature 454, 852-857 (14 August 2008) | doi:10.1038/nature07115; Received 14 April 2008; Accepted 23 May 2008; Published online 2 July 2008

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Structural basis for translation termination on the 70S ribosome

Martin Laurberg1,2, Haruichi Asahara1,2, Andrei Korostelev1,2, Jianyu Zhu1, Sergei Trakhanov1 & Harry F. Noller1

  1. Department of Molecular, Cell and Developmental Biology and Center for Molecular Biology of RNA, University of California at Santa Cruz, Santa Cruz, California 95064, USA
  2. These authors contributed equally to this work.

Correspondence to: Harry F. Noller1 Correspondence and requests for materials should be addressed to H.F.N. (Email: harry@nuvolari.ucsc.edu).

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At termination of protein synthesis, type I release factors promote hydrolysis of the peptidyl-transfer RNA linkage in response to recognition of a stop codon. Here we describe the crystal structure of the Thermus thermophilus 70S ribosome in complex with the release factor RF1, tRNA and a messenger RNA containing a UAA stop codon, at 3.2 Å resolution. The stop codon is recognized in a pocket formed by conserved elements of RF1, including its PxT recognition motif, and 16S ribosomal RNA. The codon and the 30S subunit A site undergo an induced fit that results in stabilization of a conformation of RF1 that promotes its interaction with the peptidyl transferase centre. Unexpectedly, the main-chain amide group of Gln 230 in the universally conserved GGQ motif of the factor is positioned to contribute directly to peptidyl-tRNA hydrolysis.

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