FIGURE 2. RVG-9R peptide binds and delivers siRNA to neuronal cells in vitro, resulting in gene silencing.
From the following article:
Transvascular delivery of small interfering RNA to the central nervous system
Priti Kumar, Haoquan Wu, Jodi L. McBride, Kyeong-Eun Jung, Moon Hee Kim, Beverly L. Davidson, Sang Kyung Lee, Premlata Shankar & N. Manjunath
Nature 448, 39-43(5 July 2007)
doi:10.1038/nature05901
a, Mobility of free or peptide-complexed siRNA was analysed by agarose-gel electrophoresis. b, Neuro 2a cells were examined for uptake of FITC-siRNA complexed with RVG-9R at the indicated concentrations. c, Neuro 2a and HeLa (inset) cells were examined for uptake of FITC-siRNA complexed with RVG-9R or RV-MAT-9R peptides at a 1:10 molar ratio. Lipofectamine transfection (Lipofect.) was used as a positive control. d, Neuro 2a cells stably expressing GFP were transduced with GFP siRNA complexed with RVG-9R or RV-MAT-9R peptides, and GFP silencing was tested 2 days later. A representative histogram and cumulative data from three independent experiments (inset) are shown. The grey filled histogram represents Neuro 2a cells not expressing GFP. MFI, mean fluorescence intensity. Error bars indicate s.d.
