Supplementary information

Supplementary Figures

This file contains Supplementary Figures 1–5 with Legends and detailed Supplementary Methods. The Supplementary Figures present additional data on the modified alleles used in the study as well as the scheme for Mer-Cre-Mer targeting into Runx1 locus. The Supplementary Figures also show the Runx1 expression in E7.5-E8.25 mouse concepti, the highly Runx1-positive cell clusters in day 8 - day 9 yolk sacs and provide the additional information on the analysis of the unspecific cell labeling. Supplementary Figure 5 shows the whole-mount X-Gal staining performed 12 hours after the 4'OHT administration at the nominal stage E8.0. The Supplementary Methods provide details about the Cre knock-in construct and generation of chimeric animals, induction with 4-hydroxitamoxifen, flow cytometry and cell preparations, whole-mount X-Gal staining and cryosectioning and PCR genotyping including the sequences of all primers used in this work.

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