Supplementary information

Supplementary Figures 1–10, Supplementary Table 1 and Supplementary Methods

Supplementary Figure 1 details the isolation of normal myeloid progenitor populations and transduction of GMP. Supplementary Figure 2 details the characterization of primary murine leukemias induced by transduction of GMP with an MLL-AF9 retrovirus. Supplementary Figure 3 shows a comparison of colony-forming activity between L-GMP, Lin- Kit-, and lin+ cells. Supplementary Figure 4 details the characterization of leukemias generated by introduction of 20 L-GMP into secondary recipients. Supplementary Figure 5 shows an immunophenotypic analysis of L-GMP propagated in liquid culture (AKLG cells) supplemented with IL3. Supplementary Figure 6 shows the top 50 probe sets for genes that show decreased expression in the HSC and L-GMP as compared to normal progenitors. Supplementary Figure 7 shows the self-renewal associated signature as a subset of the HSC signature: a comparison with a previously published HSC signature. Supplementary Figure 8 shows that The self-renewal associated signature is not merely a prominent portion of the HSC signature. Supplementary Figure 9 shows Mef2c RNA levels assessed by real-time PCR. Supplementary Figure 10 details a model for the development of leukemia stem cells from committed progenitors. Supplementary Table 1 gives details of the mouse transplantation data.

Supplementary Figures 1–10, Supplementary Table 1 and Supplementary Methods - Download PDF file (2.1MB)