Supplementary information
From the following article:
The depolymerizing kinesin MCAK uses lattice diffusion to rapidly target microtubule ends
Jonne Helenius, Gary Brouhard, Yannis Kalaidzidis, Stefan Diez & Jonathon Howard
Nature 441, 115-119(4 May 2006)
doi:10.1038/nature04736
Supplementary Notes
This file contains Supplementary Notes, Supplementary Methods and legends for Supplementary Videos.
Supplementary Video 1
This movie shows MCAK-induced depolymerization of microtubules. Epifluorescence images (TRITC) of immobilized microtubules were recorded with 10s intervals for 12 minutes. At 4 minutes, buffer containing 4 nM MCAK was added, resulting in the depolymerization of microtubules at 1.5 
m
min-1. Video playback is 100x real-time.
Supplementary Video 2
This movie shows MCAK–GFP molecules (green) diffusing along microtubules (red) in 1 mM ATP. 0.5 nM MCAK-GFP was used. The TIRF images (FITC) were recorded in continuous mode at 100 ms per frame and overlaid onto one static epifluorescence image (TRITC) of the microtubules. Video playback is in real-time.
Supplementary Video 3
This movie shows MCAK–GFP molecules (green) diffusing along microtubules (red) in 1 mM ADP. 0.5 nM MCAK-GFP was used. The TIRF images (FITC) were recorded in continuous mode at 100 ms per frame and overlaid onto one static epifluorescence image (TRITC) of the microtubules. Video playback is in real-time.
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