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Supplementary information

From the following article:

Structure of epsilon15 bacteriophage reveals genome organization and DNA packaging/injection apparatus

Wen Jiang, Juan Chang, Joanita Jakana, Peter Weigele, Jonathan King and Wah Chiu

Nature 439, 612-616 (2 February 2006)

doi:10.1038/nature04487

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Supplementary Methods

This file contains additional details on the methods used in this study, including purification of Epsilon15 phage, CryoEM imaging, 3-D icosahedral reconstruction 3-D non-icosahedral reconstruction and a structural analysis.

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Supplementary Figures

Supplementary Figures 1–8 with accompanying legends.

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Supplementary Movie 1

The complete structure of Epsilon15 phage showing each of the structural components including tailspikes, tail hub, portal, core, dsDNA and shell proteins based on the 20 Å reconstruction with no symmetry imposed.

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Supplementary-Movie 2

The icosahedral reconstruction of Epsilon15 phage at 9.5 Å resolution showing the locations of the alpha-helices and beta-sheets of the average capsid shell protein.

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