Letter

Nature 438, 1157-1161 (22 December 2005) | doi:10.1038/nature04300; Received 18 May 2005; Accepted 6 October 2005

Genome sequencing and analysis of Aspergillus oryzae

Masayuki Machida1, Kiyoshi Asai2, Motoaki Sano1, Toshihiro Tanaka3, Toshitaka Kumagai2, Goro Terai2,20, Ken-Ichi Kusumoto4, Toshihide Arima5, Osamu Akita5, Yutaka Kashiwagi4, Keietsu Abe6, Katsuya Gomi6, Hiroyuki Horiuchi7, Katsuhiko Kitamoto7, Tetsuo Kobayashi8, Michio Takeuchi9, David W. Denning10, James E. Galagan11, William C. Nierman12,13, Jiujiang Yu14, David B. Archer15, Joan W. Bennett16, Deepak Bhatnagar14, Thomas E. Cleveland14, Natalie D. Fedorova12, Osamu Gotoh2, Hiroshi Horikawa3, Akira Hosoyama3, Masayuki Ichinomiya7, Rie Igarashi3, Kazuhiro Iwashita5, Praveen Rao Juvvadi7, Masashi Kato8, Yumiko Kato3, Taishin Kin2, Akira Kokubun3, Hiroshi Maeda6, Noriko Maeyama3, Jun-ichi Maruyama7, Hideki Nagasaki2, Tasuku Nakajima6, Ken Oda5, Kinya Okada2, Ian Paulsen12, Kazutoshi Sakamoto5, Toshihiko Sawano3, Mikio Takahashi3, Kumiko Takase1, Yasunobu Terabayashi1, Jennifer R. Wortman12, Osamu Yamada5, Youhei Yamagata6, Hideharu Anazawa17, Yoji Hata18, Yoshinao Koide19, Takashi Komori20, Yasuji Koyama21, Toshitaka Minetoki22, Sivasundaram Suharnan23, Akimitsu Tanaka24, Katsumi Isono3, Satoru Kuhara25, Naotake Ogasawara26 & Hisashi Kikuchi3

  1. Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan
  2. Computational Biology Research Center, AIST, 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
  3. National Institute of Technology and Evaluation, Nishihara 2-49-10, Shibuya-ku, Tokyo 151-0066, Japan
  4. National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan
  5. National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan
  6. Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan
  7. The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
  8. Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan
  9. Tokyo University of Agriculture and Technology, Saiwai-cho 3-5-8, Fuchu, Tokyo 183-0054, Japan
  10. The University of Manchester, Manchester M23 9PL, UK
  11. Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, Massachusetts 02142, USA
  12. The Institute for Genomic Research, Rockville, Maryland 20850, USA
  13. The George Washington University School of Medicine, Department of Biochemistry and Molecular Biology, 2300 Eye Street NW, Washington DC 20037, USA
  14. USDA/ARS Southern Regional Research Center, 1100 Robert E. Lee Boulevard, New Orleans, Louisiana 70124, USA
  15. School of Biology, University of Nottingham, Nottingham NG7 2RD, UK
  16. Tulane University, New Orleans, Louisiana 70118, USA
  17. Kyowa Hakko Kogyo Co. Ltd, 1-6-1 Otemachi, Chiyoda-ku, Tokyo 100-8185, Japan
  18. Research Institute, Gekkeikan Sake Co. Ltd, 24 Shimotoba-koyanagi-cho, Fushimi-ku, Kyoto 612-8361, Japan
  19. Amano Enzyme Inc., 4-179-35, Sue-cho, Kakamigahara, Gifu 509-0108, Japan
  20. INTEC Web and Genome Informatics Corporation, 1-3-3 Shinsuna, Koto-ku, Tokyo 136-8637, Japan
  21. Kikkoman Corporation, 399 Noda, Noda, Chiba 278-0037, Japan
  22. Ozeki Co, Ltd., 4-9 Imadudezaike-cho, Nishinomiya, Hyogo 663-8227, Japan
  23. Axiohelix, 2-45, Aomi, Koto-ku, Tokyo 135-0064, Japan
  24. Higeta Shoyu, Co. Ltd., 2-8 Chuo-cho, Choshi, Chiba 288-8680, Japan
  25. Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan
  26. Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0101, Japan

Correspondence to: Masayuki Machida1 Correspondence and requests for materials should be addressed to M.M. (Email: m.machida@aist.go.jp). The genome sequence has been submitted to DDBJ under the accession numbers AP007150–AP007177. Reprints and permissions.

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The genome of Aspergillus oryzae, a fungus important for the production of traditional fermented foods and beverages in Japan, has been sequenced. The ability to secrete large amounts of proteins and the development of a transformation system1 have facilitated the use of A. oryzae in modern biotechnology2, 3, 4. Although both A. oryzae and Aspergillus flavus belong to the section Flavi of the subgenus Circumdati of Aspergillus, A. oryzae, unlike A. flavus, does not produce aflatoxin, and its long history of use in the food industry has proved its safety. Here we show that the 37-megabase (Mb) genome of A. oryzae contains 12,074 genes and is expanded by 7–9Mb in comparison with the genomes of Aspergillus nidulans5 and Aspergillus fumigatus6. Comparison of the three aspergilli species revealed the presence of syntenic blocks and A. oryzae-specific blocks (lacking synteny with A. nidulans and A. fumigatus) in a mosaic manner throughout the genome of A. oryzae. The blocks of A. oryzae-specific sequence are enriched for genes involved in metabolism, particularly those for the synthesis of secondary metabolites. Specific expansion of genes for secretory hydrolytic enzymes, amino acid metabolism and amino acid/sugar uptake transporters supports the idea that A. oryzae is an ideal microorganism for fermentation.

Sequencing of the A. oryzae genome was accomplished using the whole-genome shotgun (WGS) approach. The 37-Mb genome was predicted to contain a total of 12,074 genes encoding proteins with a length greater than 100 amino acid residues (see Methods). The genome was confirmed to comprise eight chromosomes (chromosomes 1–8 in decreasing size), the assignment of which is different from a previous report7 (Supplementary Table S1 and schematic drawing in Supplementary Fig. S1). Interestingly, the A. oryzae genome contained numerous stretches (1,750) of (A + T)-rich sequence (that is, >90% A + T composition in 50 nucleotides or longer), 6–9 times more than for A. fumigatus (197) and A. nidulans (308).

The A. oryzae genome is larger than those of A. fumigatus and A. nidulans by approximately 34% and 29%, respectively. Syntenic analysis of the three aspergilli revealed the presence of syntenic blocks and A. oryzae-specific blocks of sequence (lacking synteny with the two other aspergilli) in a mosaic manner throughout the A. oryzae genome (Fig. 1). Phylogenetic analysis of the three aspergilli using the whole-genome data showed that A. nidulans branched off earlier than A. oryzae and A. fumigatus5. Thus, the increase in genome size seems to be due to an A. oryzae lineage-specific acquisition of sequence, rather than loss of sequence in A. nidulans and A. fumigatus. If, on the other hand, A. nidulans and A. fumigatus are assumed to have lost 7–9Mb of sequence after branching off from their A. oryzae-like ancestor, a greater proportion of syntenic blocks would be conserved between each of them and A. oryzae than between the two. However, we observed an almost equal proportion of syntenic blocks in the three species. This suggests that the genome size differences are largely due to sequence acquisition in A. oryzae. The expansion in genome size appears to be characteristic of the organisms closely related to A. oryzae, as the estimated genome size of its close relatives A. flavus (W. Nierman, personal communication) and Aspergillus niger8 is comparable to that of A. oryzae.

Figure 1: Distribution and expression of the genes on chromosome 1.
Figure 1 : Distribution and expression of the genes on chromosome 1. Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, or to obtain a text description, please contact npg@nature.com

The blue bars at the bottom indicate the regions syntenic with A. fumigatus (AF) and A. nidulans (AN) genomes (see Methods). Non-metabolism, the genes relating to the COG categories other than metabolism; Q genes, secondary metabolism genes; Extra homologues, extra A. oryzae-specific homologues; AO-specific, A. oryzae-specific genes; Genes with EST(s), genes that have one or more corresponding EST(s); Gene density, distribution of all the predicted genes. Synteny was analysed as described in the Methods.

High resolution image and legend (25K)

Using the cluster of orthologous group (COG)9 classification, most of the gene family expansion in the A. oryzae genome as compared to A. fumigatus was found to have occurred in those predicted to have roles in metabolism (C to Q), of which those for secondary metabolism (Q) are most significantly increased (Fig. 2; see also Supplementary Table S2). No significant differences were observed in the number of genes for any other COG category in comparison with A. nidulans and A. fumigatus, except for the genes involved in defence mechanisms (V) and extracellular structures (M).

Figure 2: Comparison of relative gene numbers for each COG.
Figure 2 : Comparison of relative gene numbers for each COG. Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, or to obtain a text description, please contact npg@nature.com

The ratios of the number of genes in A. oryzae against those in A. fumigatus (AO/AF), A. nidulans (AO/AN), N. crassa (AO/NC) and S. cerevisiae (AO/SC) for each COG category9 were calculated. The ratio of the number of genes in A. nidulans against A. fumigatus (AN/AF) was also indicated. X indicates the genes without homology to any of the COG categories (see Methods). COGs with a gene number ≤5 for each species (Y, N and W) are not displayed to avoid misinterpretation derived from their possibly low reliability.

High resolution image and legend (30K)

These secondary metabolism genes are enriched in regions lacking synteny with either A. fumigatus or A. nidulans (P = 9.8 × 10-32, see Fig. 1 for chromosome 1 and Supplementary Fig. S2 for all eight chromosomes), and the genes having expressed sequence tags (ESTs) are considerably enriched in the syntenic regions (P = 4.1 × 10-134). Many more cytochrome P450 genes were observed in A. oryzae (149) compared with A. nidulans (102) and A. fumigatus (65) (Table 1). Of the polyketide synthase (PKS) genes, a specific expansion of WA-like PKS genes was observed (Supplementary Table S3). In addition, the A. oryzae genome contained a variety of homologues of trichothecene hydroxylases, isotrichodermin hydroxylases and trichodiene oxygenases, as well as pisatin demethylases that are used by plant pathogenic fungi (for example, Nectria haematococca, Fusarium spp.) for detoxification of antimicrobial agents10. This is consistent with the close phylogenetic relationship of A. oryzae with the opportunistic plant pathogen A. flavus.


Although genes predicted to be involved in the aflatoxin synthetic pathway are present in A. oryzae, no ESTs of these genes were detected except for aflJ and norA (Akao, T. et al., unpublished data), whereas ESTs for all 25 of the aflatoxin pathway genes were found in A. flavus11. A. oryzae might have been selected as a non-toxigenic strain either during the long history of its industrial use or from the beginning.

In A. oryzae, all of the COG categories related to metabolism show an expansion of gene content (Fig. 2), the highest increase of which was observed for those involved in phenylalanine/tryptophan degradation (2 and 6 in Supplementary Fig. S3) and toluene/m-cresol/p-cymene degradation (9, 11 and 12 in Supplementary Fig. S3). This was based on the analysis using the Saccharomyces cerevisiae metabolic map as a reference. BAT1 and BAT2, which contribute to the metabolism of hydrophobic amino acids lysine and serine, are also over-represented (see Supplementary Fig. S4 for the entire metabolic pathways). There is also a significant expansion in the ATP-binding cassette (ABC), the amino acid-polyamine-organocation (APC) and the major facilitator superfamily (MFS) transporter genes (Supplementary Table S4), which are concerned with multidrug resistance, transport of amino acids and transport of sugars, respectively.

Within the koji culture, A. oryzae grows on the surface of solid material such as steamed rice or ground soybean, where amino acids and sugars are deficient at the beginning. The need for A. oryzae to get access to external nitrogen sources effectively and to degrade proteins and starches seems consistent with the observed expansion of the metabolism and transporter-related gene families. Judging from the EST data, the genes for alcohol dehydrogenase, pyruvate decarboxylase and sugar transporters are typical examples of the A. oryzae genes that are transcribed most strongly (Akao, T. et al., unpublished data). The strong expression of such genes might also have been enhanced through various adaptations12 during the course of domestication.

Aspergilli possess more sensor histidine kinases (13–15) than S. cerevisiae (1) and Schizosaccharomyces pombe (3), whereas histidine-containing phosphotransfer factors and response regulators are found in similar numbers. Aspergillus histidine kinases are classified into nine families (HK1–9), of which the HK8 orthologue is absent in Neurospora crassa and the sequenced plant pathogens Cochliobolus heterostrophus, Gibberella moniliformis, Fusarium graminearum and Magnaporthe grisea. Whereas A. fumigatus, A. nidulans, N. crassa and the plant pathogens possess a single HK6 gene (Nik-1 in N. crassa) that is essential for growth in high osmotic pressure, A. oryzae has two additional homologues. Continuous culturing under high osmolarity conditions (possibly through koji cultures) may have led to A. oryzae acquiring the additional Nik-1 homologues. There are three MAPKKs and MAPKKKs in the genomes of the three Aspergillus species and N. crassa. However, whereas A. nidulans and A. fumigatus possess four MAPKs and A. oryzae five, N. crassa, F. graminearum and M. grisea possess only three. Thus, A. oryzae may possess the most complex signal transduction cascade among the four filamentous fungi.

A. oryzae has the largest expansion of hydrolytic genes among the three aspergilli (Supplementary Table S5). The genomes of A. oryzae, A. fumigatus and A. nidulans contain 135, 99 and 90 secreted proteinase genes, respectively, which constitute roughly 1% of the total genes in each genome (Supplementary Table S6). All of the proteinase genes found in A. fumigatus and/or A. nidulans have orthologues in A. oryzae except for the one encoding aminopeptidase. On the other hand, several A. oryzae proteinase genes are missing in A. fumigatus and A. nidulans. Similarly, A. oryzae possesses more secretory proteinase genes that function in acidic pH, including aspartic proteinase, pepstatin-insensitive proteinase, serine type carboxypeptidase and aorsin (Supplementary Table S6). These increases may reflect A. oryzae's adaptation to acidic pH during the course of its domestication.

The phylogenetic tree of secretory aspartic proteinases from the three aspergilli genomes (Fig. 3) shows six homologous clusters (yellow boxes) distributed on all chromosomes other than chromosome 7. Their features, including intron conservation, are similar to each other except for cluster 4, which shows the highest diversity. Each cluster contains four member genes (blue boxes), namely three orthologues from each Aspergillus species and an extra A. oryzae-specific homologue. All of the extra A. oryzae homologues are located in the A. oryzae-specific regions, whereas the orthologous clusters are located in the common regions, except for AO070319000053 of cluster 4. It is interesting to note that the clustering feature of the orthologues and extra homologues for aspartic proteinases is also conserved with the genes for carboxypeptidases (Supplementary Fig. S5a) and metalloproteinases. In contrast, the number of genes encoding intracellular enzymes (Supplementary Fig. S5b), including serine proteinases, is consistent in the three aspergilli. A similar expansion pattern was also observed for the genes for maltases (Supplementary Fig. S5c) and extracellular α-glucosidases. Besides the secretory hydrolases, some metabolic genes, including those in glucose fermentation and lysine biosynthesis, showed a similar gene expansion pattern (Supplementary Fig. S6).

Figure 3: Phylogenetic analysis of aspartic proteinases.
Figure 3 : Phylogenetic analysis of aspartic proteinases. Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, or to obtain a text description, please contact npg@nature.com

The phylogenetic relationship of aspartic proteinase homologues from the three aspergilli was analysed by the ClustalX30 program, successive unweighted pair-group method using arithmetic averages (UPGMA), and drawn by TreeView (Roderic, D. M., http://taxonomy.zoology.gla.ac.uk/rod/rod.html). Orange, blue and purple characters designate the A. oryzae, A. fumigatus and A. nidulans genes, respectively. Orthologous clusters among the three aspergilli and the clusters with an extra A. oryzae homologue are indicated by yellow and blue boxes, respectively.

High resolution image and legend (65K)

It is well known that A. oryzae has three α-amylase genes (taka-amylase genes: amyA, amyB and amyC)13 that have almost identical nucleotide sequences with only one and two mismatches in the 5′-flanking and coding regions, respectively. The amyA gene has a transposon-like element at its 5′-flanking region, and the amyB and amyC genes have highly similar nucleotide sequences spanning approximately 5kilobases (kb), including an incomplete transposon sequence at their 5′-flanking region. Phylogenetic analysis supports gene duplication to account for the expansion of the three α-amylase genes after A. oryzae branched off from the other two Aspergillus species (Supplementary Fig. S5d)—this is in clear contrast to the mode of gene expansion for the secretory proteinases mentioned above.

In contrast to the overall increase in the number of proteinases, A. oryzae has fewer glycosyl hydrolases with a cellulose-binding domain (five genes) or a starch-binding domain (glaA14) to digest insoluble cellulose or raw and granular starch, respectively (Supplementary Table S5). Apparently, no additional enzymes for accessing carbohydrates are required during fermentation in contrast to those, including knottins, found in A. fumigatus, which seems appropriate for its ecological niche of rotting vegetable matter.

Protein folding in the endoplasmic reticulum is assisted by chaperones (for example, BiP, calnexin) and foldases (three protein-disulphide isomerase family proteins and a peptidyl-prolyl cistrans isomerase). As in other fungi, however, there is no calreticulin homologue (Supplementary Table S7). Major secretory component genes, which alter the efficiency of protein secretion, were identified in all three aspergilli with an exception of the A. fumigatus SSS1 homologue (Supplementary Table S8).

In comparison to the common regions, the A. oryzae-specific regions contained 1.7 times lower density of genes homologous to those in eukaryotes other than A. fumigatus and A. nidulans. In a search for bacterial homologues, we found two genes (AO070319000101 and AO070319000102) in an A. oryzae-specific region with highest sequence similarity to those of Agrobacterium tumefaciens (AGR_L_1864 (biotin carboxylase) and AGR_L_1866, hypothetical protein genes with E-values of 0.0 and 1 × 10-119, respectively). Because the two genes are adjacently located in both A. oryzae and A. tumefaciens (Supplementary Fig. S7a), and the two A. oryzae genes reside in a ‘bacterial cluster’ (Supplementary Fig. S7b), they are suggested to have been laterally transferred.

The expansion of A. oryzae-specific homologues might be the result of genome-wide duplication, as observed in yeast. The speciation of Aspergillus was estimated to have taken place approximately 20million years ago15 and was later than the whole-genome duplication event in yeast, which was estimated to have taken place 150million years ago16. We were unable to observe any extended stretch of region within the A. oryzae genome that showed a certain degree of similarity to another stretch of region despite the fact that we observed synteny among the three aspergilli (Fig. 1) and that segmentally duplicated stretches were detected by the same method within the S. cerevisiae genome. Thus, the increase in the genome size of A. oryzae relative to A. fumigatus and A. nidulans does not appear to be due to chromosomal duplication. The large segmental duplication, if any, must have taken place much earlier than the separation of the three aspergilli, and the similarity between the duplicated regions might have been completely lost by extensive sequence alterations and rearrangements. However, if the three aspergilli had a common ancestor possessing the expanded gene families found in A. oryzae, both A. nidulans and A. fumigatus must independently have lost approximately 3,000 genes in common with the putative common ancestor.

The mosaic structure of the genome, considered to be evidence for horizontal gene transfer17, was found by synteny analysis of the A. oryzae genome and was further characterized by the localization of the EST expression (see above) of non-metabolic genes (P = 1.78 × 10-95 and 1.32 × 10-51 for information and storage (J to B) and cellular function and signalling (D to O), respectively) and the genes of high codon adaptation index (top 5% genes, P = 9.8 × 10-28). The phylogenetic distance between the genes in the orthologous cluster and the A. oryzae-specific ones was similar to that between the genes of Aspergillus and the other genera belonging to Sordariomycetes. The statistical analysis by ref. 18 of some A. oryzae-specific homologues of aspartic proteinase, carboxypeptidase, maltase, pyruvate decarboxylase and lysine-ketoglutarate reductase/saccharopine dehydrogenase showed P-values of between 0.000 and 0.004. The results indicated phylogenetic inconsistency of these genes. These results, together with the above discussion, imply that the A. oryzae-specific genes have been transferred by a similar mechanism observed for an asexual pathogenic fungus19, in which chromosomes are transferred between genetically isolated clonal lines. It has been reported that yeast chromosomes are rearranged frequently under starved culture conditions and that (A + T)-rich sequences or transfer RNA often mediate such rearrangements20. Our EST analysis shows that the expression profile in solid-state cultivation is similar to that observed when a carbon source is omitted21. These reports suggest that the acquired foreign DNA has been rearranged in a short period of time by large-scale solid-state cultivation since A. oryzae was domesticated from an ancestor of A. flavus22.

It is tempting to speculate that the gene expansion of A. oryzae is explained by horizontal gene transfer; however, at this moment we cannot exclude the possibility of massive gene loss in the two other Aspergillus species. Future comparative analyses with more closely related species would provide more insight into the scenario of the genome evolution of A. oryzae, including that which occurred during the centuries of domestic cultivation.

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Methods

Strain and DNA preparation

Aspergillus oryzae RIB40 (National Research Institute of Brewing Stock Culture and ATCC-42149) was used as the DNA donor. Genomic DNA preparation and removal of mitochondrial DNA was performed as described by refs 23 and 24, respectively.

Genome sequencing

The genome sequencing of A. oryzae was accomplished using the WGS approach by accumulating raw sequence reads of approximately ×9 depth of coverage. Contigs generated were mapped by Southern hybridization onto chromosomes separated by PFGE. Linkage between contigs was analysed by fingerprinting and PCR methods. Sequence assembly was validated with high-density end sequences of bacterial artificial chromosome (BAC) and cosmid clones and by Optical Mapping (OpGen). See Supplementary Information for details.

Gene prediction and annotation

Genes were predicted in the A. oryzae genome based on the homologies to known genes in the public database, ESTs of A. oryzae and A. flavus, and the statistical features of the genes by applying a combination of gene-finding software. Transfer RNAs were identified using tRNAScan-SE25. Repeated sequences were detected using RepeatMasker (Smit, A. F. A. and Green, P., http://ftp.genome.washington.edu/RM/RepeatMasker.html). The homologues of the proteins of aspergilli, N. crassa, M. grisea, Gibberella zeae, Penicillium and Paecilomyces are searched for by running BlastX with a threshold value of E ≤ 1 × 10-10. The resultant candidates of homologues are evaluated by ALN26, which predicts the precise gene structures by aligning the Blast hits and the protein sequences. ALN takes into account frameshift errors, coding potentials and signals for translational initiation, termination and splicing. Of the 6,586 genes thus predicted by ALN, 489 highly reliable genes were adopted into a learning set for GeneDecoder27 and GlimmerM28 software that work based on the statistic features of genes. GeneDecoder also integrates the information for splice sites provided by the ESTs, which are aligned with the genome sequence by SIM4 (ref. 29). Fivefold cross-validation of the gene finders trained by the above data set showed sensitivity/specificity for the exon prediction of 0.74/0.53 and 0.66/0.59 for GeneDecoder and GlimmerM, respectively, and those for coding sequences of 0.93/0.90 and 0.92/098. Genes partially supported by ESTs were predicted by GeneDecoder and those without any support by the known genes or ESTs were predicted by GlimmerM. The numbers of genes predicted by ALN, GlimmerM and GeneDecoder were 5,367, 6,983 and 1,713, respectively. All of the predicted protein-coding genes were annotated by searching against the COG database9 using BlastP, followed by manual corrections.

Synteny analysis

Orthologues between A. oryzae and either A. nidulans or A. fumigatus were identified using the best bi-directional hit method (BlastP with a bit score greater than 200). In addition, putative homologous regions between the species were identified by TBlastX with a bit score greater than 100. Orthologues and homologous regions between the contigs of two species were aligned to make a contiguous block, until no orthologues or homologous regions were found within the range of 10kb. A region of conserved synteny was defined as the longest contiguous block that contained at least one orthologue and one additional orthologue or homologous region.

COG analysis

The number of genes for each COG category was analysed by a BlastP search using the amino acid sequences in the COG set9 with the bit score of ≥60.

Gene localization

Distribution of all predicted genes and the genes with ESTs that were obtained from mycelia grown in either liquid-rich medium, liquid-starved medium or solid-state cultivation (Akao, T. et al., unpublished data) were analysed by counting the corresponding genes in a 5-kb window. Distributions of non-metabolic genes, secondary metabolism genes, extra A. oryzae-specific homologues that have homology (bit score ≥100) to orthologues identified by best bi-directional match between A. oryzae and either A. fumigatus or A. nidulans, as well as A. oryzae-specific genes without homology to either A. fumigatus or A. nidulans genes (bit score <100) were analysed in the same way by applying a window size of 15kb.

Statistical analyses

Localization of the secondary metabolism genes at the A. oryzae-specific regions was evaluated by the one-tailed P-value based on the binomial distribution with the sample size of 413. Localization of the genes with EST expression, non-metabolic genes and the top 5% of genes with a high CAI value at the syntenic regions was evaluated in the same way with sample sizes of 33,77, 1,839 and 703, respectively. The analyses were performed when A. oryzae-specific regions were detected by comparing the A. oryzae and A. fumigatus genomes. The phylogenetic inconsistency was statistically analysed by the method described in ref. 18 using data sets consisting of the genes of the three aspergilli and three species belonging to Sordariomycetes or Eurotiomycetes other than Aspergillus. The reference and test data sets included the A. oryzae gene in the orthologous cluster and the extra A. oryzae-specific homologue, respectively.

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References

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Supplementary Information

Supplementary information accompanies this paper.

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Acknowledgements

The authors are grateful to N. Hall and H. Hagiwara for discussions and critical reading of the manuscript. We thank M. Tadenuma and T. Ishikawa of the Brewing Society of Japan for the office work necessary for the collaborative research work of companies, national institutes and universities. Author Contributions M.S., T.T., K. Kusumoto, T.A., Y. Kashiwagi, H. Horikawa, A.H., R.I., Y. Kato, A.K., N.M., T.S., K.T., S.S., K. Isono, S.K., N.O., H.K. and M.M. sequenced the A. oryzae genome; the genes were computationally predicted and annotated from the A. oryzae genome by K.A., T. Kumagai, G.T., J.Y., D.B., T.E.C., O.G., T. Kin, H.N. and T. Komori; M.S., K. Kusumoto, T.A., O.A., Y. Kashiwagi, K.A., K.G., H. Horiuchi, K. Kitamoto, T. Kobayashi, M. Takeuchi, D.W.D., D.A., J.W.B., M.I., K. Iwashita, P.R.J., M.K., H.M., J.M., T.N., K. Oda, I.P., K.S., Y.T., O.Y., Y.Y., H.A., Y.H., Y. Koide, Y. Koyama, T.M., A.T. and M.M. contributed correction and hand annotation of the predicted genes; K.A., T. Kumagai, G.T., D.W.D., J.E.G., W.C.N., N.D.F., T. Kin, H.N., Y.T., J.W., T. Komori and M.M. analysed gene localization and development of the A. oryzae genome.

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Competing interests statement

The authors declare no competing financial interests.

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