Supplementary information
From the following article:
Calcium triggers exit from meiosis II by targeting the APC/C inhibitor XErp1 for degradation
Nadine R. Rauh, Andreas Schmidt, Jenny Bormann, Erich A. Nigg & Thomas U. Mayer
Nature 437, 1048-1052(13 October 2005)
doi:10.1038/nature04093
Supplementary Figure S1
Excess IVT-XErp1WT or -XErp1T195A blocks calcium-induced CSF release demonstrating that both proteins are active and folded properly. The stability of 35S-labelled IVT-securin and the morphology of the chromatin was used to monitor the cell cycle stage of the extract.
Supplementary Figure S2
XErp1 is degraded in Xenopus 
90 extracts possesing CaMKII activity.
Supplementary Figure S3
CaMKII triggers Plx1-dependent degradation of XErp1. Endogenous XErp1 is rapidly degraded in CSF extract supplemented with constitutively active CaMKII or calcium. CaMKII induced degradation of XErp1 depends on Plx1 activity as indicated by the fact that a dominant negative acting form of Plx1 (PBD) prevents CaMKII triggered XErp1 destruction.
DOWNLOAD BROWSER PLUGINS AND OTHER APPLICATIONS
Movie files
PDF douments
Text documents
PostScript documents
Flash movies
Audio files
- Apple iTunes (PC or Mac)
- QuickTime Player (PC or Mac)
- Realplayer (PC or Mac)
- Windows Media player (PC only)
